摘要
目的观察元胡止痛滴丸对痛经模型大鼠的镇痛作用,并探讨其可能的作用机制。方法选取雌性SPF级Wistar大鼠60只,按照体重随机区组的方法将其分为对照组,模型组,元胡止痛滴丸小(0.2 g生药/kg)、低(0.4 g生药/kg)、中(0.8 g生药/kg)、高(1.6 g生药/kg)剂量组,每组各10只。腹腔注射缩宫素致痛诱发大鼠形成痛经模型,腹腔注射苯甲酸雌二醇致敏后连续灌胃给药3 d,观察元胡止痛滴丸对痛经引起的扭体反应的潜伏期和扭体次数的影响,并检测血浆中前列腺素F_(2α)、前列腺素E_2等与疼痛相关因子的水平。结果与模型组比较,元胡止痛滴丸低、中、高剂量组大鼠的扭体次数明显减少(P <0.05或P <0.01),扭体潜伏期有延长趋势,但由于个体差异导致标准差较大,差异无统计学意义(P> 0.05)。与模型组比较,元胡止痛滴丸中、高剂量组大鼠血浆中β内啡肽及前列腺素E_2含量显著升高(P <0.05或P <0.01),去甲肾上腺素、5羟色胺及前列腺素F_(2α)/前列腺素E_2比值显著降低(P <0.05或P <0.01),元胡止痛滴丸高剂量组大鼠前列腺素F2α含量明显降低(P <0.05);元胡止痛滴丸中、高剂量组大鼠血浆中血栓素B_2、6-酮前列腺素F_(1α)含量与模型组比较差异无统计学意义(P> 0.05)。结论元胡止痛滴丸在0.8～1.6 g生药/kg剂量下对痛经模型大鼠具有显著的镇痛作用,改善外周血中与疼痛相关的前列腺素、单胺类递质及β-内啡肽水平是其发挥多途径镇痛作用的可能机制。
Objective To study the analgesic effect of Yuanhu Zhitong Dropping Pills on dysmenorrhea model rats and to discuss the possible mechanism. Methods Sixty female SPF grade Wistar rats were divided into control group, model group, small-dose （0.2 g crude drug/kg）, low-dose （0.4 g crude drug/kg）, medium-dose（0.8 g crude drug/kg）, high-dose（1.6 g crude drug/kg） of Yuanhu Zhitong Dropping Pills group according to weight random block method, with 10 rats in each group. The model of dysmenorrhea was induced by intraperitoneal injection of oxytocin, and Yuanhu Zhitong Dropping Pills was administered by intragastric administration for 3 days after sensitized by intraperitoneal injection of estradiol benzoate. The effect of Yuanhu Zhitong Dropping Pills was observed by detecting latency stage and times of writhing response induced by dysmenorrhea, and the level of factors related to pain such as prostaglandin F2α and prostaglandin E2 in plasma were also detected. Results Compared with model group, the times of writhing response in low-dose , medium-dose, high-dose of Yuanhu Zhitong Dropping Pills group significantly decreased （P 〈 0.05 or P 〈 0.01）, and the preclinical period of writhing response in low-dose, medium-dose, high-dose of Yuanhu Zhitong Dropping Pills group showed an extension trend, while the standard deviation was large due to individual differences, the differences were not statistically significant （P 〉 0.05）. Compared with model group, the level of β-endorphin and prostaglandin E2 in medium-dose and high-dose of Yuanhu Zhitong Dropping Pills group increased significantly （P 〈 0.05 or P 〈 0.01）, and the level of noradrenaline, 5-hydroxytryptamine, the ratio of prostaglandin F2α to prosta-glandin E2 decreased significantly （P 〈 0.05 or P 〈 0.01）, the level of prostaglandin F2α in high-dose of Yuanhu Zhitong Dropping Pills group decreased significantly （P 〈 0.05）. There was no statistically significant difference on the level of thromboxane B2 and 6-Keto-prostaglandin F1α between medium-dose, high-dose of Yuanhu Zhitong Dropping Pills group and model group （P 〉 0.05）. Conclusion Yuanhu Zhitong Dropping Pills has significant analgesic effect on dysmenorrhea model rats with the dose of 0.8-1.6 g crude drug/kg. The multi-way analgesic effect is probably related to improved levels of pain-associated prostaglandins, monoamine transmitters, and β-endorphin in peripheral blood.
作者
冯玥
张宗鹏
申秀萍
胡金芳
FENG Yue;ZHANG Zongpeng;SHEN Xiuping;HU Jinfang(Tianjin Institute of Pharmaceutical Research New Drug Evaluation Co.,Ltd.,Tianjin,300301,China;Tianjin Engineering Research Center of Drug Preclinical Assessment Technology,Tianjin,300301,China)
出处
《中国医药导报》
CAS
2018年第33期8-12,共5页
China Medical Herald
基金
国家科技重大专项项目(2015ZX09501004-002-003)
天津市科技计划项目(16PTGCCX00090)
天津市科技计划项目(17ZXXYSY00020)
关键词
元胡止痛滴丸
镇痛
痛经
缩宫素
Yuanhu Zhitong Dropping Pills
Analgesia
Dysmenorrhea
Oxytocin
