摘要
基于胡椒转录组数据库,筛选胡椒病程相关基因非表达子基因的EST序列,设计引物,运用RACE法克隆得到1个NPR基因,命名为PnNPR2;该基因全长2 260 bp,开放阅读框1 722 bp,编码573个氨基酸;开放读码框含有BTB/POT结构域、ANK锚蛋白重复序列、DUF和NPR1-like C 4个结构域,具有植物NPR1所共有的保守结构域,并将PnNPR2基因插入pCAMBIA1300-35S-sGFP超表达载体中。本研究结果为PnNPR2的功能研究奠定基础。
The full-length cDNA encoding nonexpressor of pathogenesis-related genes（NPR）, designated PnNPR2, was isolated from black pepper by using RACE. The sequence of PnNPR2 was 2 260 bp in length, containing a 1 722 bp open reading frame, and encoding a polypeptide of 573 amino acids. The protein had conserved domains of BTB/POT, ANK anchored protein repeat sequences, DUF and NPR1-like C. The recombinant plasmid pCAMBIA1300-PnNPR2 was obtained by inserting the PnNPR2 gene into the pCAMBIA1300 vector. The results showed that the recombinant plasmid pCAMBIA1300-PnNPR2 which contained the target gene was constructed successfully. The result would generate an important theoretical and practical significance for black pepper genetic improvements.
作者
范睿
胡丽松
伍宝朵
郝朝运
FAN Rui;HU Lisong;WU Baoduo;HAO Chaoyun(Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wanning,Hainan 571533,China;Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops,Ministry of Agriculture,Wanning,Hainan 571533,China;Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops,Wanning,Hainan 571533,China)
出处
《热带作物学报》
CSCD
北大核心
2018年第10期1983-1989,共7页
Chinese Journal of Tropical Crops
基金
国家自然科学基金(No.31601626)
中国热带农业科学院基本科研业务费(No.1630142017015)
关键词
胡椒
NPR2
克隆
抗病
black pepper
nonexpressor of pathogenesis-related genes 2 （NPR2）
cloning
disease resistance
