摘要
为探究微肥与氮磷钾肥不同配比量对川泽泻主要药效成分含量的影响,本研究以川泽泻为试验材料,采用四因素五水平二次正交旋转组合设计进行田间试验。通过高效液相色谱(HPLC)法测定泽泻主要药效成分23-乙酰泽泻醇B(Y_1)、24-乙酰泽泻醇A(Y_2),建立主要药效成分与锌(X_1)、硼(X_2)、钼(X_3)和氮磷钾肥(X_4)施用量的数学函数模型。结果表明,所建立的回归方程均达到显著水平。通过对所建立数据模型进行统计频数的选优可知,当锌肥为17.685 0～20.272 5 kg·hm^(-2)、硼肥为24.890 0～28.034 5 kg·hm^(-2)、钼肥为0.192 3～0.270 4 kg·hm^(-2)、氮肥为309.920 0～355.940 0 kg·hm^(-2)、磷肥为166.88～191.66 kg·hm^(-2)、钾肥为202.64～232.73 kg·hm^(-2)时,23-乙酰泽泻醇B的含量大于0.11%。当锌肥为17.820～20.265 kg·hm^(-2)、硼肥为23.826～26.842 kg·hm^(-2)、钼肥为0.179～0.199 kg·hm^(-2)、氮肥为333.970～389.090 kg·hm^(-2)、磷肥为179.830～209.510 kg·hm^(-2)、钾肥为218.365～268.539 kg·hm^(-2)时,24-乙酰泽泻醇A的含量大于0.15%。综上,本研究优化出了提高川泽泻中24-乙酰泽泻醇A、23-乙酰泽泻醇B含量的施肥方案,且结果可靠,为泽泻生产上提高药效品质提供了理论依据。
Different ratios of N, P and K fertilizers were studied to explore the effects of chemical profile in Alisma plantago-aquatica Linn, a field experiment was carried out using four factors and five level quadratic orthogonal rotation combination design. Alisol B 23-acetate（Y1） and Alisol A 24-acetate（Y2） were determined by HPLC, medical composition and a mathematical function model of the fertilization rate of zinc（X1）, boron（X2）, molybdenum（X3） and NPK 26：14：17（X4） was established. The results showed that the mathematical regression model reached significant level. According to the mathematical regression model, when the zinc fertilizer was 17.685～20.272 5 kg·hm^-2 with boron fertilizer of 24.890 0～28.034 5 kg·hm^-2, molybdenum fertilizer of 0.192 3～0.270 4 kg·hm^-2, nitrogen of 309.92～355.94 kg·hm^-2,phosphate of 166.88～191.66 kg·hm^-2, and potash of 202.64～232.73 kg·hm^-2, the concentration of Alisol B 23-acetate was up to 0.11%. Additionally, the concentration of Alisol A 24-acetate was over 0.15% when the zinc fertilizer was 17.820～20.265 kg·hm^-2 with the boron fertilizer of 23.826～26.842 kg·hm^-2, molybdenum fertilizer of 0.179～0.199 kg·hm^-2, nitrogen of 333.970～389.090 kg·hm^-2,phosphate of 179.830～209.510 kg·hm^-2, and potash of 218.365～268.539 kg·hm^-2. This experiment optimizes the fertilization scheme for improving the content of 24-acetyl Alisol A and 23-acetyl Alisol B in Alisma plantago-aquatica Linn. The results were accurate,and provided a theoretical basis for improving the content of medicinal ingredients in Alisma plantago-aquatica Linn.
作者
窦明明
雷飞益
石峰
马留辉
张亚琴
杨正明
陈雨
陈兴福
DOU Mingming;LEI Feiyi;SHI Feng;MA Liuhui;ZHANG Yaqin;YANG Zhengming;CHEN Yu;CHEN Xingfu(Key Laboratory of Crop Physiology,Ecology and Cultivation in Southwest,Ministry of Agriculture/ Agronomy College of Sichuan Agricultural University,Chengdu,Sichuan 611130)
出处
《核农学报》
CAS
CSCD
北大核心
2018年第12期2462-2470,共9页
Journal of Nuclear Agricultural Sciences
基金
四川省育种攻关项目(2011NZ0098-12-01)
关键词
川泽泻
配方施肥
二次正交旋转
药效成分
Alisma plantago-aquatica Linn.
formula fertilization
quadratic orthogonal rotating combination design
medicinal composition
