摘要
目的探讨-20℃冻存条件下分装冻融对SD大鼠血清生化检测结果的影响。方法仪器经校准、质控合格后,采集40只空白雌性SD大鼠血液,2～8℃静止30 min后离心,血清分装并用封口膜封口,每只动物分装4份。室温4 h的血清检测分析结果作为基准参考水平,其余3份血清放置于-20℃冷冻保存,分别于2 d、7 d、14 d复融后检测,比较室温4 h与-20℃冷冻保存各保存时间对生化检测结果的影响。结果-20℃冻存条件下分装冻融14 d血清生化检测结果与室温4 h比较TP、ALB、GLU、CREA可见统计学差异(P<0.05);分装冻融7 d血清生化检测结果与室温4 h比较ALT、TP、ALB、GLU、UREA、CREA、TC可见统计学差异(P<0.05);分装冻融2 d血清生化检测结果与室温4 h比较ALT、TP、ALB、GLU、CREA可见统计学差异(P<0.05);分装冻融2 d、7 d、14 d血清生化检测结果与室温4 h比较AST、TG均未见统计学差异(P>0.05)。结论-20℃分装冻融2 d、7 d、14 d血清生化检测结果与室温4 h比较,AST、TG检测结果稳定未见影响,其余七项检测指标均可见明显影响,本实验可知如无特殊情况尽量减少血清分装冻融的次数,建议最好当天检测,否则会影响检测结果的准确性。
Objective To investigate the effect of sub packaged freezing and thawing on serum biochemical test in SD rats at-20 ℃ for cryopreservation. Method After the instrument was calibrated and quality control was passed, the blood of 40 blank female SD rats was collected, stored for 30 min at 2～8 ℃, then centrifuged, the serum was dispensed and sealed with a sealing film, and each animal was dispensed in 4 portions. Serum detection analysis was done under room temperature for 4 h and the result was the reference level. The remaining 3 sera were stored at-20 ℃ for cry preserved and were re-thawed and detected after 2 d,7 d,14 d respectively. The effects of biochemical test result at room temperature for 4 h were compared with those at-20 ℃ for cryopreservation for different time. Result There were statistical differences of the concentration of TP, ALB, GLU and CREA in the serum biochemical test result between the condition of freezing-thawing at-20 ℃ for 14 d and room temperature for 4 h（P〈0.05）. There were statistical differences of the concentration of ALT, TP, ALB, GLU, UREA, CREA and TC in the serum biochemical test result between the condition of freezing-thawing at-20 ℃ for 7 d and room temperature for 4 h（P〈0.05）. There were statistical differences of the concentration of ALT, TP, ALB, GLU and CREA in the serum biochemical test result between the condition of freezing-thawing at-20 ℃ for 2 d and room temperature for 4 h（P〈0.05）.There was no statistical difference of the concentration of AST and TG in the serum biochemical test result between the condition of freezing-thawing at-20 ℃ for 2 d, 7 d, 14 d and room temperature for 4 h（P〉0.05）. Conclusion The serum biochemical test result of AST and TG were stable and consistent between the condition of freezing and thawing at-20 ℃ for 2 d, 7 d, 14 d and room temperature for 4 h while the other seven result have changed. This experiment shows that minimize the number of serum freeze-thawing as few as possible if there is no special circumstance, and suggests that it is best to detect in that day otherwise it will affect the accuracy of the test result.
作者
孙昌华
刘娜
王春芳
张娟
祝清芬
SUN Changhua;LIU Na;WANG Chunfang;ZHANG Juan;ZHU Qingfen(Shandong Institute for Food and Drug Control,Jinan 250101,China)
出处
《实验动物科学》
2018年第5期52-55,共4页
Laboratory Animal Science
关键词
分装冻融
SD大鼠
血清
sub packaged freezing-thawing
SD rats
serum
