摘要
【目的】考察棘腹蛙(Rana boulengeri)白细胞介素-34(Interleukin-34,IL-34)基因的信息和该基因组织表达特性。【方法】基于实验室构建的棘腹蛙皮肤转录组数据库,采用反转录PCR从棘腹蛙皮肤组织中克隆IL-34基因的全长cDNA序列,并用荧光定量PCR法检测IL-34基因在棘腹蛙各组织中的表达情况。【结果】棘腹蛙IL-34基因的cDNA全长为864bp,包含564bp的开放阅读框,编码187个氨基酸;预测IL-34理论分子量大小为70.56kDa,理论等电点为4.85。荧光定量PCR分析表明IL-34基因在棘腹蛙皮肤、脑、肝脏、脾脏、胃、肌肉等组织中均有表达,在脾脏组织中表达量最高。【结论】成功克隆了棘腹蛙IL-34基因并探明了它的组织表达模式,为深入研究棘腹蛙IL-34的生物学功能提供了理论依据。
[Purposes]To investigate gene information and tissue expression characteristics of gene of interleukin-34（IL-34）in Rana boulengeri.[Methods]The complete open reading frame（ORF）sequence of IL-34 was amplified by RT-PCR according to the transcriptome of R.boulengeri skin constructed by our laboratory.The tissue expression analysis was used by qRT-PCR in main tissues.[Findings]The results indicated that the full length cDNA sequence of R.boulengeri IL-34 consist of 864 bp,containing an ORF of 564 bp.The putative IL-34 is predicted to be a peptide of 187 amino acids,with a calculated molecular weight of 70.56 kDa and an isoelectric point of 4.85.The analysis of fluorescence quantitative PCR showed that IL-34 was expressed in the skin,brain,liver,spleen,stomach and muscle tissues,and the highest expression was expressed in the spleen tissues.[Conclusions]IL-34 from R.boulengeri was cloned successfully and the tissue distribution was analyzed,which provides a reference for the biological function of the late IL-34 dig.
作者
刘静
景晓玲
罗洁
李晓英
樊汶樵
LIU Jing;JING Xiaoling;LUO Jie;LI Xiaoying;FAN Wenqiao(Institute of Aquatic Animal Disease Prevention and Control,College of l.ife Science ＆ Forestry,Chongqing University of Art ＆ Science,Yongchuan Chongqing 402168;State Key Laboratory of Silkworm Genome Biology,Southwest University,Chongqing 400715,China)
出处
《重庆师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第6期29-33,共5页
Journal of Chongqing Normal University:Natural Science
基金
重庆市基础科学与前沿技术研究重点专项(No.cstc2015jcyjBX0013)
重庆市社会事业与民生保障科技创新专项科技扶贫一般项目(No.cstc2017shms-kjfp80006)
重庆市教育委员会科学研究项目(No.KJ1601123
No.KJ1601109)
重庆文理学院人才引进专项(No.R2013LS14)
重庆文理学院校级重点项目(No.2017ZLX20)
关键词
棘腹蛙
白介素-34
克隆
组织表达
Rana boulengeri
interleukin-34
cloning
tissue expression
