摘要
目的探索玻璃化冷冻保存技术对体外成熟(in vitro maturation,IVM)卵母细胞发育潜能和转录组的影响。方法选择2014年9月—2016年3月期间在北京大学第三医院生殖医学中心接受腹腔镜手术联合经阴道穿刺取卵行卵母细胞IVM治疗的不孕症患者为研究对象进行前瞻性队列研究,将研究对象分为IVM组(A组,n=13)和IVM-冻卵组(B组,n=24)。分析卵母细胞的早期胚胎发育状况和单细胞水平的转录组学情况。结果 IVM后玻璃化冷冻-解冻对存活卵母细胞的早期胚胎发育没有明显影响,受精率、卵裂率、优质胚胎率和可移植胚胎率组间差异均无统计学意义。但IVM后行玻璃化冷冻-解冻对转录组存在一定影响,与A组相比,B组共有1 913个基因呈现差异表达,其中570个基因表达量升高,主要富集到59个生物学过程;1 343个基因表达量降低,主要富集到140个生物学过程,而且一些差异基因参与了卵母细胞质量、受精和胚胎发育潜能的调控。结论 IVM后行玻璃化冷冻-解冻对早期胚胎发育没有明显影响,但对转录组具有一定影响,且发生表达量变化的基因与卵母细胞质量、受精和胚胎发育潜能有关,提示玻璃化冷冻-解冻有可能影响子代安全性。
ObjectiveTo investigate the effect of vitrification on the development potential and transcriptome of in vitro matured human oocytes.
MethodsThis is a prospective cohort study. The infertility patients treated by laparoscopic surgery combined with in vitro maturation （IVM） treatment were analyzed in Center for Reproductive Medicine of Peking University Third Hospital, between September 2014 and March 2016. The research subjects were divided into two groups： IVM group （group A, n=13） and IVM-vitrification group （group B, n=24）. The early embryonic development and transcriptome at the single cell level of the oocytes from two groups were compared and analyzed.
ResultsThe process of oocytes vitrification-thawing after IVM had no significant effect on the early embryonic development. There was no significant difference in survival oocyte fertilization rate, cleavage rate, advanced embryo rate and transferable embryo rate between the two groups. However, vitrification-thawing after IVM had a certain effect on transcriptome. Compared with group A, 1 913 genes in group B showed differential expression, of which 570 genes were upregulated and mainly enriched to 59 biological processes while 1 343 genes were downregulated and mainly enriched to 140 biological processes. Furthermore, some differentially expressed genes and their related biological processes were involved in the regulation of oocyte quality, fertilization and embryonic development potential.
ConclusionThe process of oocyte vitrification-thawing after IVM had no significant effect on early embryonic development, but had some effects on transcriptome. These changed genes were related to oocyte quality, fertilization and embryo development potential, suggesting that vitrification-thawing may affect the offspring safety.
作者
霍莹
覃清圆
卢翠玲
宋雪凌
郑晓英
于洋
闫丽盈
廉颖
刘平
李蓉
严杰
乔杰
Huo Ying;Qin Qingyuan;Lu Cuiling;Song Xueling;Zheng Xiaoying;Yu Yang;Yan Liying;Lian Ying;Liu Ping;Li Rong;Yan Jie;Qiao Jie(Key Laboratory of Assisted Reproduction,Ministry of Education,Beijing Key Laboratory of Reproductive Endocrinology andAssisted Reproduction,Beijing 100191,China;Center for Reproductive Medicine,Department of Obstetrics and Gynecology,Peking University Third Hospital,Beijing 100191,Chin;Peking-Tsinghua Center for Life Sciences,Peking University,Beijing 100871,Chin;Department of Physiology and Pathophysiology,Basic Medical College,Peking University,Beijing 100191,Chin;National Clinical Research Center of Obstetrics and Gynecology,Beijing 100191,China)
出处
《中华生殖与避孕杂志》
CAS
CSCD
北大核心
2018年第10期789-796,共8页
Chinese Journal of Reproduction and Contraception
基金
国家重点研发计划(2017YFC1002002)
国家自然科学基金(81571386
31230047
31429004)
中华医学会临床医学科研专项基金(16020280644)~~
