摘要
目的提高海洋细菌Bacillus cereus CAMT2377产葡萄糖氧化酶活力。方法对碳源、氮源、无机盐等培养基组成及装液量、接种量、培养温度、发酵时间、转速等培养条件进行筛选,通过单因素分析和统计优化提升菌株产酶性能。结果 Bacillus cereus CAMT2377产葡萄糖氧化酶适宜培养基成分为1%麦芽糖、0. 5%鱼粉蛋白胨、0. 05%CaCl_2,在此培养基中接种8%体积比的种子液后,于31℃,20%装液量,200 r/min条件下培养60 h,产葡萄糖氧化酶活力可在10 U/mL以上;响应面优化产酶过程获得最适参数为温度35℃,时间82 h,转速247 r/min,优化条件下产酶活力提高至15 U/mL以上,较优化前提高了50%。结论通过单因素分析和响应面优化,提高了海洋细菌Bacillus cereus CAMT2377产葡萄糖氧化酶的活力。
Objective To enhance the glucose oxidase production by marine Bacillus cereus CAMT2377. Methods One-factor-one-time analysis and statistical optimization procedure were applied to investigate the enzyme production,by which the components of medium,such as carbon source,nitrogen source and inorganic salt,as well as culture condition including volume of culture medium,inoculation amount,culture temperature,fermentation time and rotation rate,were screened. Results The optimal medium for glucose oxidase production consisted of 1% maltose,0. 5% fish meal and 0.05% calcium chloride. The optimal volume of culture medium,inoculation amount,culture temperature,fermentation time and rotation rate were 20%,8%（volume ratio）,31 ℃,60 h and 200 r/min respectively. Under the optimal condition,the activity of glucose oxidase was more than 10 U/mL. Furthermore,response surface method was applied to optimize the glucose oxidase production,by which the optimal temperature,time and rotation rate were 35 ℃,82 h and247 r/min respectively. The activity of glucose oxidase under the condition optimized by response surface method increased to more than 15 U/mL,which increased by 50% as compared with that before optimization. Conclusion The glucose oxidase production by Bacillus cereus CAMT2377 was enhanced by optimization of culture condition by onefactor-one-time analysis and response surface method.
作者
黄晓月
莫日坚
徐德峰
叶日英
孙力军
王雅玲
HUANG Xiao-yue;MO Ri-jian;XU De-feng;YE Ri-ying;SUN Li-jun;WANG Ya-ling(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Guangdong Provincial Engineering Technology Research Center of Marine Food,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution,Zhanjiang 524088,Guangdong Province,China)
出处
《中国生物制品学杂志》
CAS
CSCD
2018年第11期1268-1273,共6页
Chinese Journal of Biologicals
基金
国家自然科学基金项目(31201309
31371777)
广东省科技厅项目(2013B021100016
2014B020205006)
广东省教育厅项目(2014-KTSCX078
2013050205
2013050203
2013050312)
农业部东海与远洋渔业资源开发利用重点实验室开放课题(2014K03)
广东海洋大学大学生创新课题(CXXL2014042)
关键词
海洋细菌
葡萄糖氧化酶
优化
Marine bacterium
Glucose oxidase
Optimization
