摘要
目的探讨高频电疗对急性脊髓损伤(ASCI)大鼠神经功能恢复和脑源性神经营养因子-酪氨酸蛋白激酶受体B(BDNF-TrkB)表达的影响。方法选取成年雌性SD大鼠75只,随机分为S1、S2、S3、S4、S5五组各15只,S1组仅行椎板切除术暴露硬脊膜,不予打击,S2、S3、S4组在脊髓损伤造模后1 d对受损部位给予无热量短波、超短波、微波治疗至取材前,S5组不给予任何治疗。比较各组损伤后1d、1周、2周、3周神经功能评分(Zea-Longa评分法),记录在损伤后不同时段BDNF、TrkB表达水平,同时分析其与Zea-Longa评分相关性。结果损伤各组各时点Zea-Longa评分均高于S1组(P<0.05),各时点S2、S3组Zea-Longa评分低于S4、S5组,1周时S3组Zea-Longa评分低于S2组,2周、3周时S4组Zea-Longa评分低于S5组(P<0.05);损伤1周时各组BDNF、TrkB细胞阳性数达高峰,且S3组最高,S2组次之,S4、S5组低于S2、S3组(P<0.05),随后均下降,2周时S2、S3组高于S4、S5组,S3组高于S2组(P<0.05),3周时S2、S3、S4组高于S5组,S3组高于S2组、S4组(P<0.05);相关性分析显示ASCI大鼠Zea-Longa评分与BDNF、TrkB阳性细胞数呈负相关(P<0.05)。结论高频电疗尤其是短波、超短波可明显提高脊髓损伤大鼠神经功能恢复程度,损伤后1周超短波效果最明显,微波疗效较弱,可能与上调BDNF、TrkB表达水平有关。
Objective To explore the effects of high-frequency electrotherapy on the recovery ofneurological function and the expression of brain-derived neurotrophic factor tyrosine kinase receptorB （BDNF-TrkB） in rats with acute spinal cord injury （ASCI）. Methods A total of 75 adult female SDrats were selected and randomly divided into the S1, S2, S3, S4 and S5 groups （n=15）. The S1 groupwas treated with laminectomy to expose the spinal dura mater but no spinal cord injury, and the S2,S3, S4 groups were given the non -heat shortwave, ultrashort wave and microwave treatmentrespectively to the damaged sites at 1d after the formation of spinal cord injury model until the drawingmaterial, and the S5 group was not given treatment. The neurological function score （Zea-Longa score）at 1 day, 1 week, 2 weeks and 3 weeks after injury, was compared among the groups, and theexpression levels of BDNF, TrkB were recorded at different time points after injury, and the correlationwith Zea-Longa score was analyzed. Results The Zea-Longa score in each injured group was higherthan that in the S1 group （P〈0.05）, and the Zea-Longa score in the S2 and S3 groups was lower thanthat in the S4 and S5 groups, and the Zea-Longa score in the S3 group at 1 week was lower than thatin the S2 group, and the Zea-Longa score in S4 group at 2 weeks and 3 weeks was lower than that inthe S5 group（P〈0.05）. At 1 week after injury, the positive numbers of BDNF and TrkB cells were up tothe peak in each group, and the highest was in the S3 group followed by the S2 group, and the levelsin the S4 and S5 groups were lower than those in the S2 and S3 groups, and then they were alldecreased, and the levels in S2 and S3 groups were lower than those in S4 and S5 groups at 2 weeks,and the levels in the S3 group were lower than those in the S2 group （P〈0.05）. At 3 weeks, the levelsin the S2, S3 and S4 groups were higher than those in the S5 group, and the levels in the S3 groupwere higher than those in the S2 and S4 groups （P〈0.05）. Correlation analysis showed that the Zea-Longa score of ASCI rats was negatively correlated with the positive numbers of BDNF and TrkB （P〈0.05）. Conclusions High-frequency electrotherapy, especially short-wave and ultrashort wave, cansignificantly improve the recovery of neurological function in rats with spinal cord injury. The effect ofultrashort wave is the most obvious at 1 week after injury, and the microwave effect is weak, whichmay be related to the up-regulation of BDNF and TrkB expression.
作者
周刚
王佳君
ZHOU Gang;WANG Jia-jun(Department of Neurology,Huanggang Central Hospital,Huanggang,Hubei,438000,China)
出处
《颈腰痛杂志》
2018年第6期694-697,共4页
The Journal of Cervicodynia and Lumbodynia
关键词
高频电疗
急性脊髓损伤
大鼠
神经功能恢复
BDNF
TrkB、
high-frequency electrotherapy
acute spinal cord injury
rats
neurological functionrecovery
BDNF
TrkB
