摘要
目的建立反相高效液相色谱法(RP-HPLC)测定忧遁草中夏佛塔苷含量,比较两个产地的忧遁草中夏佛塔苷的含量。方法采用Diamonsil C18(2)色谱柱(250 mm×4.6 mm,5μm),流动相A相为0.1%乙酸水溶液,B相为甲醇,等度洗脱:0～60 min 24%B,流量1 ml/min,柱温30℃,检测波长272 nm。结果夏佛塔苷在5.88～15.68μg范围内与峰面积呈良好的线性关系(R2=1);平均加样回收率为95.49%,RSD为1.61%。结论该方法简单,准确,重复性好,国产忧遁草中夏佛塔苷的含量明显地高于印度尼西亚忧遁草。
Objective To establish a RP-HPLC method for determination of schaftoside in Clinacanthus Nutans （Burm. F.） Lindau and compare the content of schaftoside in Clinacanthus Nutans （Burm. F.） Lindau from two origins. Methods A Diamonsil C18 （2） column （250 mm×4.6 mm, 5 μm） was adopted, with the temperature set at 30 ℃. The mobile phase consisted of 1 % acetic acid （A） and methanol （B）. The isocratic elution was used according to the following profle： 0-60 min, 24 % B. The detection wavelength was 272 nm. Results The calibration curve of schaftoside had a good linearity within 5.88-15.68 μg （R2=1）. The average recovery was 95.49 %, with RSD of 1.61 %. Conclusion The method is simple and accurate, with excellent repeatability. The content of chataroside in Clinacanthus Nutans （Burm. F.） Lindau from China is signifcantly higher than that in Indonesia.
作者
刘梦
林强
LIU Meng;LIN Qiang(Beijing Union University,Beijing 100191,China)
出处
《食品与药品》
CAS
2018年第6期425-428,共4页
Food and Drug
关键词
忧遁草
夏佛塔苷
产地
反相高效液相色谱法
Clinacanthus Nutans （Burm. F.） Lindau
schaftoside
origin
RP-HPLC
