摘要
运用末端限制性片段长度多态性T-RFLP法(terminal restriction fragment length polymorphism),检测岩溶区典型13种灌丛植物根系丛枝菌根(arbuscular mycorrhizal,AM)群落结构变化特征,探讨岩溶区AM与宿主植物是否存在选择特性.结果表明,不同灌丛植物根际土壤养分存在显著差异,豆科植物显著高于非豆科植物.聚类分析表明,13种灌丛植物均能被AM侵染,不同灌丛植物根系AM群落结构存在显著差异,尤其豆科与非豆科植物,但AM群落结构在9种非豆科植物间差异显著而在4种豆科植物间则差异不显著.冗余分析表明,影响豆科与非豆科植物根系AM群落结构的因子各异,土壤Olsen-P、p H和全氮是影响岩溶区灌丛植物根系AM群落组成结构的主要因子.研究还表明,岩溶区灌丛植物根系AM与宿主植物具有选择特性,这种选择特性出现在植物功能群的几率比植物种类大,将AM应用于岩溶区植物恢复时,应考虑AM与宿主植物的选择特性问题.
To explore if there are species-preferential characteristics of arbuscular mycorrhizal （AM） and host plants in karst regions, 13 shrub plants （including leguminosae and non-leguminosae） were selected to study the AM community structure of root samples. The soil nutrients in rhizosphere soils significantly differ among shrubs; they are higher in leguminosae than in non-leguminosae. Cluster analysis shows that all 13 shrubs can be infected by AM. Significant differences of the AM community structure were observed among root samples from different shrubs, especially leguminosae and non-leguminosae. Redundancy analysis shows that soil Olsen-P, pH, and total nitrogen significantly influence the AM community structure of plant roots, although the factors affecting this fungus in leguminosae and non-leguminosae differ. These results indicate species-preferential characteristics of AM and host plants in karst regions, especially of the plant function group compared with plant species, suggesting that these characteristics should be taken into account when AM fungi are used for vegetation restoration in karst regions.
作者
梁月明
苏以荣
何寻阳
陈香碧
LIANG Yue-ming;SU Yi-rong;HE Xun-yang;CHEN Xiang-bi(Key Laboratory of Karst Dynamics,Ministry of Nature and Resources ＆ Guangxi,Institute of Karst Geology,Chinese Academy of Geological Sciences,Guilin 541004,China;Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtrooical A~ricuhure.Chinese Ac~rlemv nf Sei~ne~ C,hnn~,~hn zl.lfHg~ Ch nn)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2018年第12期5657-5664,共8页
Environmental Science
基金
国家应急管理项目(31741021)
中国科学院西部之光人才培养计划"西部青年学者"项目(Y62305040)
广西自然科学基金项目(2016GXNSFBA380179
2017XNSFAA198241)
岩溶地质研究所基本科研业务费项目(2016004)
中国地质大调查项目(DD20160324)
关键词
岩溶
灌丛
豆科植物
丛枝菌根真菌
群落结构
karst
shrub
leguminosae
arbuscular mycorrhizal fungus
community structure
