摘要
论述和分析了影响苹果高效离体再生的主要因素 ,包括试验材料苹果的基因型、外植体生理状态、培养基类型、植物激素、Ag NO3、前期暗培养、抗生素等内外因子 ,以及影响根癌农杆菌介导苹果遗传转化的主要因素 ,包括苹果基因型、外植体生理状态、菌株类型、菌液浓度、活化物质的应用、共培养、预培养、选择培养、培养基成分等内外因子。并对其研究现状。
The paper summarizes and analyses main influencing factors of highly efficient regenerations in vitro including apple genotype,explants properties,plant hormone ratio and level,AgNO 3,dark incubation,antibiotics,et al,and influencing factors of transformations mediated by Agrobacterium tumefaciens including apple genotypes,explants properties Agrobacterium strain, Agrobacterium concentration,function of activated matters co cultivation,pretreatment,selection culture,medium types,et al.The present situation of its studies,existing problems and its prospects are briefly mentioned.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2002年第4期135-140,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金资助项目 (3 9970 5 2 2 )
关键词
根癌农杆菌
介导
苹果
遗传转化
apple
Agrobacterium tumefaciens
genetic transformation
influencing factors
