摘要
研究了水环境中不同Ca2 + 浓度 (2 0、35、6 0、80和 15 0mg·L-1)、盐度 (7、14和 2 0‰ )和 pH(7 6、8 8和 9 8)对日本沼虾 (Macrobrachiumnipponense)肝胰腺中消化酶 (胃蛋白酶和类胰蛋白酶 )和碱性磷酸酶的影响 .结果表明 ,Ca2 + 对日本沼虾的胃蛋白酶有促进作用 ,Ca2 + 浓度为 15 0mg·L-1时酶活力最高 ;高浓度的Ca2 + 对类胰蛋白酶有抑制作用 ,而在一定范围内 ,碱性磷酸酶活力随Ca2 + 浓度的增高而增高 .盐度为 14‰时 ,日本沼虾的胃蛋白酶、类胰蛋白酶和碱性磷酸酶活力均高于 7‰和 2 0‰组 .随着 pH值升高 ,蜕皮率和 3种酶活力也随之增高 ,pH9 8时均达到最高值 ,但增长率和增重率则降低 .
The activities of digestive enzymes and alkaline phosphatase from the hepatopancreas of Macrobrochium nipponense were determined under different environmental factors (calcium concentrations 20 mg·L -1 ,35 mg·L -1 ,60 mg·L -1 ,80 mg·L -1 ,150 mg·L -1 ;salinity 7‰,14‰,20‰;pH7 6,8 8,9 8).The results showed that higher Ca 2+ concentration could enhance the pepsin activity,but inhibit the trysin-like activity in hepatopancreas of M.nipponense.The activities of pepsin,trysin-like,alkaline phosphatase in hepatopancreas of M.nipponense were higher under salinity of 14‰ than under salinity of 7‰ and 20‰.It showed that the activities of digestive enzymes and alkaline phosphatase of shrimp increased gradually with increasing pH value from 7.6 to 9.8.
出处
《应用生态学报》
CAS
CSCD
2002年第9期1153-1156,共4页
Chinese Journal of Applied Ecology
基金
河北省重点科技攻关资助项目(96220502D)
关键词
环境因子
日本沼虾
消化酶
碱性磷酸酶
Macrobrochium nipponense,Digestive enzymes,Alkaline phosphatase,Calcium,Salinity,pH.
