摘要
设计6组实验,测定在3种不同饲料蛋白质水平(44%,48%,52%)下添加2种水平的小肽(0.05%,0.1%)对赤点石斑鱼(Epinephelus akaara)生长、消化酶、血清生化和抗氧化能力的影响。饲料分别为D1(44%,0.05%),D2(44%,0.1%),D3(48%,0.05%),D4(48%,0.1%),D5(52%,0.05%),D6(52%,0.1%)。每组饲料3个平行,每个玻璃钢纤维桶(500 L)放15尾石斑鱼[初始体质量(42.16±0.23)g],实验进行8周。结果显示,各组石斑鱼增重、特定生长率、肌肉粗蛋白、脂肪和灰分无显著差异,随着蛋白质和小肽水平的增加,肌肉粗脂肪、血清胆固醇和甘油三酯质量浓度降低(P<0.05);胃蛋白酶活性在D2组最高,小肽对肠道淀粉酶、胰蛋白酶和脂肪酶活性不存在显著影响,在D1组活性较高(P>0.05);各组石斑鱼肝脏丙二醛和过氧化物酶差异不显著(P>0.05)。由上述结果可以看出在不同蛋白质水平下添加小肽可以降低石斑鱼的蛋白需求,促进脂肪代谢,增加消化酶活性。
Six diet groups containing protein( 44%,48%,52%) and small peptide( 0. 05% and 0. 1%) were fed to groupers( Epinephelus akaara) to assess the effect on growth performance,digestive enzymes activities,serum biochemical and antioxidant abilities.The diets were named as D1( 44%,0. 05%), D2( 44%,0. 1%), D3( 48%,0. 05%), D4( 48%,0. 1%), D5( 52%,0. 05%) and D6( 52%,0. 1%). Each diet was fed to triplicate groups of fish with 15 fish [initial weight( 42. 16 ± 0. 23) g] whichwere randomly sorted into each rearing cubic fiberglass tanks and the experimental last for eight weeks. The results show that there was no effect on weight gain( WG),specific growth rate( SGR),muscle moisture,crude protein and ash contents. As protein level and small peptide concentration increased,the crude fats of grouper muscle,triglyceride( TG) and cholesterol( CHO) decreased( P < 0. 05).The activity of pepsin was the highest in Group D2,and the activities of amylase,trypsin and lipase were the highest in Group D1.There were no significant difference among different groups( P > 0. 05). The activities of malondialdehyde( MDA) and peroxidase( POD) were not significantly different among different groups( P >0. 05). The result shows that supplementation of small peptide at different protein levels can reduce protein requirement,promote fat metabolism and increase digestive enzymes activities.
出处
《南方水产科学》
CAS
CSCD
北大核心
2016年第3期15-23,共9页
South China Fisheries Science
基金
公益性行业(农业)科研专项经费项目(201403011)
关键词
石斑鱼
小肽
饲料蛋白质
生长
血清生化
抗氧化能力
Epinephelus akaara
small peptide
dietary protein
growth
serum biochemical indices
antioxidant abilities
