摘要
为了确定乌苏里拟鲿(Pseudobagrus ussuriensis)体表溃疡症的病因,在无菌操作下从发病鱼的溃疡灶边缘、脾、肾中分离病原菌;利用湿片法对鳃组织、体表黏液和肠道内容物压片,观察寄生虫寄生;利用病理学技术观察病鱼的病理损伤特点,分析病因。结果发现,采用BHI培养基作为该病例的首次分离培养基效果不佳,未见细菌生长。该病损伤的主要靶器官为皮肤肌肉、脾脏、头肾和体肾,所有鱼均主要表现为溃疡性坏死性皮炎和脂膜炎,脾炎和间质性肾炎。还可见轻微的灶性坏死性鳃炎。眼、脑、肝脏、心脏、消化道、鳔等未见明显病理改变。将病变组织的石蜡切片进行吉姆萨染色,在肌肉组织内可发现大量细长、可弯曲,犹如"乱发样"细菌。此外,在病灶内还可见少量真菌菌丝。综上结果,判定该病为细菌和真菌共同感染引起。证明了病理学技术在该病诊断中的重要作用。
To determine the etiology of Pseudobagrus ussuriensis with skin ulcer,bacteriology was conducted from the edge of ulcer,the spleen and the kidney. Besides,wet mounts from gill,skin mucus and intestinal content were checked to exclude parasites. Pathological characteristics,target organs and visible pathogens were determined by histopathology. The results show that BHI is not a good medium for the first time bacteria isolation and culture from fish. No obvious bacteria were seen on the medium. The target organs were skin and muscle,spleen,head kidney and trunk kidney. All the fish mainly expressed necrotizing ulcerative dermatitis and panniculitis,splenitis and interstitial nephritis. Moreover,mild focal branchitis was also found. No obvious change was obversed in eye,brain,liver,heart,digestive tract and gas bladder. After re-staining the slides with Gimsa,we found long filamentous and hair-like bacteria in muscle tissue. Furthermore,fungal hyphae were also seen in muscle tissue. The etiology of this case was considered a coinfection of bacteria and fungi. The findings support the importance of histopathology in diagnosis.
出处
《南方水产科学》
CAS
CSCD
北大核心
2016年第3期106-111,共6页
South China Fisheries Science
基金
四川省科技支撑计划项目(2014NZ0027)
2014校级教学改革与研究项目(X2014001)
关键词
病理学诊断
乌苏里拟鲿
溃疡症
pathological diagnosis
Pseudobagrus ussuriensis
ulcer
