摘要
对青稞(Hordeum vulgare)褐穗病菌(Corticium rolfsii)的生物学特性及营养利用能力进行了研究。结果表明,青稞褐穗病菌担孢子萌发的温度范围为5~30℃,最适温度为20℃;在自由水中可以萌发,但在100%相对湿度中不萌发;pH为4.53~9.18时均可萌发,最适pH为5.59;48h萌发率为76.5%;蔗糖、土壤浸渍液、青稞叶浸渍液和牛羊粪浸渍液4种营养液及光照处理对孢子萌发没有明显的促进作用。担孢子在温度15.5~21.0℃、相对湿度31%~71%下,最少能存活69d。果糖培养基褐穗病菌菌丝生长显著高于对照(P【0.05),山梨糖、淀粉和乳糖有显著的抑制作用,硝酸铵和硫酸铵对褐穗病菌菌丝生长略有促进作用,但差异不显著(P】0.05),而谷氨酸、酪氨酸、尿素和碳酸氨对菌丝生长有显著抑制作用。
In the present paper,the biological characteristics and nutrition utilization ability of Corticium rolfsii isolated from Hordeum vulgare were studied.The basidiospore of C.rolfsii can germinate at temperature range from 5to 30℃and pH range from pH 4.53to pH 9.18.The optimal germination conditions were 20℃and pH 5.59which had 76.5% germination ratio after 48hours.The basidiospore can germinate in free water,however,it cannot germinate at relative humidity 100%.The basidiospore germination rates did not significantly improve under both nutrient solutions including sucrose,soil and leaf and light treatment.Under 15.5~21.0℃and relative humidity 31%~71%conditions,the basidiospore still can germinate even after 69days.The mycelia of C.rolfsii grew normally on media with carbon source such as starch,glucose,sucrose,fructose,cellulose,maltose,lactose,sorbitol,galactose,mannose,and xylose and nitrogen source such as urea,tyrosine,ammonium nitrate,threonine,ammonium carbonate,ammonium sulfate and glutamate.The mycelia of C.rolfsii grow grew slowly on media with sorbose and ammonium carbonate.
出处
《草业科学》
CAS
CSCD
北大核心
2014年第7期1248-1253,共6页
Pratacultural Science
关键词
青稞
褐穗病
生物学特性
营养利用
Hordeum vulgare
Corticium rolfsii
biological characteristics
nutrition utilization ability
