摘要
建立了鸡肝组织中磺胺嘧啶、磺胺二甲基嘧啶、磺胺甲氧吡嗪、磺胺 6 甲氧嘧啶、磺胺甲恶唑、磺胺 2 ,6 二甲氧嘧啶和磺胺喹恶啉的多残留高效液相色谱分析法。样品经乙腈提取、正己烷液 液分配和碱性氧化铝柱固相萃取法净化后用反相高效液相色谱法测定 (UV2 70nm)。 7种磺胺类药物标准曲线的相关系数 (r2 )为 0 9991～1 0 0 0 0 (0 0 1μg/ml～ 0 5 μg/ml)。在 0 0 5mg/kg～ 0 5mg/kg浓度范围内 ,磺胺类药物的回收率为 6 8 8%～10 0 0 % ,变异系数为 1 3%～ 12 0 %。检测限为 0 0 1mg/kg～ 0 0 3mg/kg ,定量限低于 0 0 5mg/kg。
A method for simultaneous determination of seven sulfonamides in chicken liver is presented in this paper.The samples were extracted with acetonitrile,and cleaned up by n-hexane liquid-liquid extraction (LLE) and basic alumina solid-phase extraction (SPE).The drugs were determined by reversed-phase high perfomance liquid chromatography (RP-HPLC) with UV absorbance detection at 270 nm.The calibration curves for seven sulfonamides were linear in a concentration range of 0.01-0.05 μg/ml(r 2=0.9991-1.0000).The recoveries ranged from 68.8% to 100.0% at 0.05-0.5 mg/kg fortified samples,with coefficients of variation of 1.3%～12.2%.The limits of detection were 0.01-0.03 mg/kg,and the limits of quantification less than 0.05 mg/kg,in 5.00 g sample.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2002年第5期468-472,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金 ( 39870 5 85 )
农业部专项资助
