摘要
对细胞融合技术获得的1株新型融合微藻(Tetraselmissp. 1)进行摇瓶混合培养,并分析光照强度对该培养条件下融合微藻的影响。结果表明:1)在培养基中添加葡萄糖对融合微藻的生长有强烈的促进作用,融合微藻生长速率为0.576g/(L·d),是自养培养的7.38倍;2)在光照强度为3000lx混合培养时,葡萄糖消耗速率达最大值3g/(L·d);3)在光照强度为2000lx混合培养时,藻细胞密度最大,为2.60g/L,以葡萄糖计算的基质得率随光照强度的增加而显著下降;4)融合微藻叶绿素a含量在混合培养条件下,随光照强度的增大而增加;5)在混合培养条件下,光照对NH4+的同化为正影响,融合微藻氮含量和CC/CN分别为5.68和6.06,均介于自养培养与异养培养之间。
<Abstrcat> A novel strain of cell fused microalgae Tetraselmis sp. 1 was obtained from Pavlova viridis and Tetraselmis sp. . Under different light intensity and with different culture medium, the Tetraselmis sp.1 was incubated. The results show that: 1) glucose has obvious effects on growth of Tetraselmis sp.1 and the growth rate is 0.576 g/(L·d) ,which is 7.38 times as much as that under autotrophic culture ; 2)at 3 000 lx of light intensity ,the glucose consuming rate is the highest,3 g/(L·d) ; 3)at 2 000 lx of light intensity ,the cell density gets to the maximum of 2.60 g/L, and the biomass/glucose yield decrease with the increase of light intensity; 4)the contents of chlorophylla increase with the increase of light intensity; 5) the assimilation of NH4+ is positively influenced by light intensity,and the content of nitrogen and the ratio of carbon to nitrogen are 5.68 and 6.06, respectively, which are both between those of autotrophic culture and heterotrophic culture.
出处
《中国水产科学》
CAS
CSCD
北大核心
2002年第3期239-242,共4页
Journal of Fishery Sciences of China
基金
国家"八六三"高技术发展资助项目(863-819-Q-17)
关键词
光照强度
细胞融合藻
混合培养
生长
有机碳源
light intensity
cell fused microalgae (Tetraselmis sp.-1)
mixotrophic cultivation
