摘要
Objective: To examine the expression and localisation of adrenomedullln in hum an coronary atherosclerotic lesions from patients with unstable angina(UAP) and stable angina(SAP), and to study the relation between adrenomedullin expression and plaque instability. Design: A retrospective observational study. Patients: D irectional coronary atherectomy samples were obtained from 15 patients with UAP and 12 with SAP. Methods: The localisation of adrenomedullin was examined by imm unohistochemistry, and adrenomedullin mRNA expression was measured by quantitati ve polymerase chain reaction. Results: Adrenomedullin immunoreactivity was prefe rentially localised in macrophages, intimai smooth muscle cells, and proliferate d microvessels. The mean number of adrenomedullin positive cells in five high po wer fields(x 400) per specimen was higher in patients with UAP than in those wit h SAP (mean (SEM), 110(13) v 76 (7); p< 0.05); and the ratio of adrenomedullin p ositive to total cells was higher in patients with UAP (43.0 (2.2)%v 34.2 (2.0) %; p< 0.01). More adrenomedullin mRNA was expressed in the plaque of patients w ith UAP than in those with SAP (60.4 (16.9)%v 9.7 (3.3)%; p< 0.01). Conclusion s: The findings suggest that adrenomedullin is involved in the development of at herosclerosis and plaque instability in human coronary arteries, in an autocrine or paracrine manner.
Objective: To examine the expression and localisation of adrenomedullln in hum an coronary atherosclerotic lesions from patients with unstable angina(UAP) and stable angina(SAP), and to study the relation between adrenomedullin expression and plaque instability. Design: A retrospective observational study. Patients: D irectional coronary atherectomy samples were obtained from 15 patients with UAP and 12 with SAP. Methods: The localisation of adrenomedullin was examined by imm unohistochemistry, and adrenomedullin mRNA expression was measured by quantitati ve polymerase chain reaction. Results: Adrenomedullin immunoreactivity was prefe rentially localised in macrophages, intimai smooth muscle cells, and proliferate d microvessels. The mean number of adrenomedullin positive cells in five high po wer fields(x 400) per specimen was higher in patients with UAP than in those wit h SAP (mean (SEM), 110(13) v 76 (7); p< 0.05); and the ratio of adrenomedullin p ositive to total cells was higher in patients with UAP (43.0 (2.2)%v 34.2 (2.0) %; p< 0.01). More adrenomedullin mRNA was expressed in the plaque of patients w ith UAP than in those with SAP (60.4 (16.9)%v 9.7 (3.3)%; p< 0.01). Conclusion s: The findings suggest that adrenomedullin is involved in the development of at herosclerosis and plaque instability in human coronary arteries, in an autocrine or paracrine manner.
