摘要
以戊二醛为交联剂 ,将葡萄糖氧化酶 (GOD)和介体甲苯胺蓝 (TBO)通过共价键作用固定于铂电极表面上的溶胶 -凝胶薄膜载体上 ,制成了葡萄糖生物传感器 ;该功能化薄膜由γ_氨丙基甲基二甲氧基硅烷和四甲氧基硅在稀盐酸催化作用下通过水解 -缩聚反应制得 ;红外光谱证实 ,GOD和TBO在载体上的固定化是通过交联剂分子上的醛基与GOD、TBO和载体上的氨基反应实现的 ,共价交联方式有效地避免了酶和介体从载体上的脱落 ;葡萄糖生物传感器的循环伏安特性表明 ,TBO能在GOD反应活性中心与铂电极表面之间进行有效的电子传递;葡萄糖生物传感器对底物葡萄糖响应的线性范围是1～8mmol/L,检出限是0.05mmol/L。
An amperometric glucose biosensor has been fabricated by immobilizing glucose oxidase(GOD) along with the mediator toluidine blue O (TBO) to a functional sol-gel thin layer on the surface of a platinum electrode via the cross-linking agent glutaraldehyde. The functional sol-gel thin layer was prepared through the hydrolysis and polycondensation of γ-aminopropylmethyldimethoxysilane and tetramethoxysilane in a dilute hydrochloric acid medium. The IR spectra showed that GOD and TBO were covalently linked to the carrier sol-gel material through the interaction between the aldehyde groups in glutaraldehyde and the amino groups in GOD, TBO and their carrier. Covalent immobilization prevents possible shedding of the sensing reagent and mediator from the carrier. Cyclic voltammetry was employed to demonstrate that TBO is capable to transfer electrons effectively between the active sites of the immobilized GOD and the surface of a platinum electrode. The calibration curve of glucose is linear in the range of 1～8 mmol/L, with a detection limit of 0.05 mmol/L. The lifetime of the biosensor exceeds one month.
出处
《分析测试学报》
CAS
CSCD
北大核心
2002年第5期23-26,共4页
Journal of Instrumental Analysis
基金
西安交通大学科学研究基金资助项目 (XJJJ200020)
