摘要
目的 通过大鼠在不同发育阶段中胃底腺细胞表达α—N—乙酰氨基葡萄糖 (GluNAc)残基的情况 ,来研究大鼠胃底腺颈粘液细胞的发生。方法利用单克隆抗体HIK10 83能特异性识别α—GluNAc残基的特点 ,进行免疫组化研究。结果HIK10 83阳性细胞从第 19 5天胚胎鼠的胃底腺中开始辨认出来 ,一直存在至成鼠。这些细胞最初出现在胃底腺的底部 ,然后向上方移动 ,最后位于腺体的颈部。结论胚胎晚期开始表达α—GluNAc残基的细胞可能是原始的颈粘液细胞 ,随着鼠胃底腺的发育 ,这些细胞逐渐向上方移动 。
Objective To investigate the ontogeny of mucous neck cells by observing α linked N acetyglucosamine(α GlcNAc) residue expression in the developing rat fundic gland Method Immunohistochemical method was applied using a monoclonal antibody, HIK 1083, which could specifically recognizeα GlcNAc residues Result In the developing rat fundic gland,cells expressing α GlcNAc residues were discerned from day 19 5 of gestation and continued to exist during the development These cells were located at the bottom of the fundic gland when they first appeared With the elongation of the gland, these cells moved upwards and were finally restricted in the neck region of the gland Conclusion Mucous cells expressing α GlcNAc residues in the late embryo period mighit be primary mucous neck cells With the development of the fundic gland, these cells would gradually move upwards and finally become mucous neck cells
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2002年第3期279-281,354,共4页
Chinese Journal of Histochemistry and Cytochemistry
