摘要
目的研究中药制剂尿毒清片的质量标准。方法采用薄层色谱扫描法对尿毒清片中大黄、何首乌、苦参、丹参、川芎等成分进行了色谱鉴别;采用薄层色谱扫描法测定了方中黄芪甲苷的含量;采用高效液相色谱法测定了方中芍药苷的含量。结果该方法专属性强,含量测定黄芪甲苷在1.006～9.054μg间、芍药苷在0.176～0.880μg间呈现良好的线性关系,黄芪甲苷回收率为97.69%,RSD=2.13%,芍药苷回收率为97.47%,RSD=0.87%。结论该方法可作为尿毒清片的质量控制方法。
Objective: To study the quality standard of Niaoduqing Tablet, a preparation of Chinese herbal medicine. Methods: Thin layer chromatography (TLC) was used for the identification of Radix et Rhizoma Rhei, Radix Polygoni Multiflori, Radix Sophorae Flavescentis, Radix Salviae Miltiorrhizae and Rhizoma Chuanxiong in Niaoduqing Tablet. TLC scanning was used for the determination of astragaloside IV and HPLC for determination of paeoniflorin in Niaoduqing Tablet. Results: TLC identification were highly specific. A good linearity for astragaloside IV was ranged 1.006～9.064μg and that for paeoniflorin was ranged 0.176～0.880μg. The average recovery of astragaloside IV was 97.69 %( RSD= 2.13 %) and that of paeoniflorin was 97.47 %( RSD=0.87 %). Conclusion: This method can be used for the standard of quality control of Niaoduqing Tablet.
出处
《中药新药与临床药理》
CAS
CSCD
2002年第5期302-305,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
关键词
质量标准
尿毒清片
黄芪甲苷
芍药苷
高压液相色谱法
薄层色谱扫描法
Niaoduqing Tablet/chemistry
@Astragaloside /analysis
@ Paeoniflorin/analysis
Chromatography , thin layer
Chromatography , high pressure liquid
