摘要
目的 探讨氟影响学习记忆功能的作用机理。方法 应用 3H- DL -谷氨酸为示踪剂进行突触小体谷氨酸摄取实验 ,通过体内、体外实验 ,观察氟对大鼠脑突触小体谷氨酸摄取功能的影响。结果 1.0～ 4 0 .0mm ol/ L剂量的氟化钠在体外作用 15 m in,能使海马区突触小体 3H- DL -谷氨酸摄取量抑制 18.95 %～ 75 .6 3% ,大脑皮层谷氨酸摄取量抑制 2 5 .91%～ 80 .31% ,且存在明显的剂量 -效应关系。动物经 2 0 .0 ,4 0 .0 mg· kg- 1 · d- 1氟化钠饮水染毒 5 0 d,然后直接进行摄取实验 ,海马区突触小体谷氨酸摄取功能被抑制了 19.85 %和 4 1.2 1% ,后者与对照组相比差异有显著意义 ,而大脑皮层谷氨酸摄取量虽然也被抑制 5 .6 2 %和 2 0 .4 3% ,但与对照组相比没有统计学意义。结论 氟可抑制谷氨酸递质的摄取灭活作用。
Objective In order to investigate the mechanism of effect of fluoride on learning and memory.Methods An experiment of glutamate uptake in the cerebral cortex and hippocampus synaptosome of rat was carried out in vivo and in vitro.Results In vitro, fluoride(1.0~4.0 mmol/L) could inhibit 3H DL glutamate uptake both in cerebral cortex and hippocampus synaptosome by 18.95%~75.63% and 25.91%~80.31%, respectively, with a dose response pattern. high dose fluoride(≥10.0 mmol/L) could significantly reduce 3H DL glutamate uptake. In vivo, hippocampus synaptosome uptake was apparently inhibited after exposure to 40 mg·kg -1 ·d -1 of NaF for 50 days.Conclusions Fluoride can decrease the uptake of glutamate in rat brain synaptosome.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2002年第5期354-356,共3页
Chinese Jouranl of Endemiology
基金
卫生部科研基金资助项目 (96-2 -2 0 4)
