摘要
目的 :探讨煤焦沥青 (CTP)烟气诱发小鼠肺癌组织中黑素瘤抗原 - a(mage- a)基因 m RNA的表达及其意义。 方法 :CTP烟气诱发小鼠肺癌 ,提取癌组织中 RNA ,用逆转录 -巢式聚合酶链反应 (RT- PCR)方法对癌组织和相应癌旁组织 mage- am RNA表达情况进行研究 ;p UC18质粒克隆 ,大肠杆菌 DH5α转化 ;PE- 377DNA测序仪对 2个阳性克隆中的目的基因片段进行 DNA序列测定。结果 :实验组 2 9只小鼠中 8只诱发出肺癌 (8/ 2 9) ;8只小鼠肺癌中 ,有 5只癌组织 mage- a m RNA表达阳性(5 / 8) ;相应的癌旁组织均未表达。 DNA序列测定证明扩增产物中目的基因片段均为 m age- a c DNA序列。结论 :小鼠肺癌组织中 m age- a m RNA呈高比例表达 ,CTP烟气诱发小鼠肺癌可能是 MAGE- A抗原肺癌免疫治疗的理想模型。
Objective: To investigate the expression of mage-a mRNA in lung cancer tissues of mice induced by coal tar pitch(CTP) fume and to discuss the above model for lung cancer immunotherapy with mage-a. Methods: Tumor tissue samples of lung cancer and paired non-tumor tissues were obtained from 8 lung cancer mice. Total RNA was extracted and cDNA was synthesized. Nested polymerase chain reaction amplification using mage-a specific primer was performed to detect the expression of mage-a. The 2 clones of mage-a mRNA positive PCR products were sequenced by DNAs sequencer (PE-377). Results: Of 29 mice in the experimental group, 8 were induced to lung cancer.Among which 5 (5/8) expressed mage-a mRNA. The expression of mage-a gene was not found in adjacent lung tissues. The DNA sequencing confirmed that the target gene fragments in 2 samples of PCR products were mage-a cDNA. Conclusion: The mage-a gene is highly expressed in lung cancer in mice induced by CTP fume, suggesting that CTP-induced lung cancer in mice may be an ideal animal model for lung cancer therapeutic experiment with MAGE-A.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2002年第9期1007-1010,共4页
Academic Journal of Second Military Medical University
基金
河南省高校杰出科研人才创新工程基金资助项目 ( 2 0 0 0 KYCX0 0 4)
河南省自然科学基金资助项目( 0 1110 2 14 0 0 )
河南省分子医学重点实验室资助项目
