摘要
目的 研究白细胞介素 12 (IL 12 )、IL 13在哮喘发病中的作用。方法 用逆转录聚合酶链反应 (RT PCR)法半定量分析了哮喘急性发作期及正常对照组儿童外周血单个核细胞 (PBMC)中IL 12、IL 13mRNA表达水平的变化 ,同时对IgE水平进行了检测。结果 哮喘组IL 12mRNA表达减少 ,IL 13mRNA表达增多 ;病情越重 ,IL 12mRNA表达越少 ,IL 13mRNA表达越多 ;无论IgE升高与否 ,与对照组比较 ,IL 12、IL 13mRNA表达 ,差异均有显著性。结论 IL 12、IL 13可能是构成气道慢性炎症的各类因素之一。
Objective To study the role of IL 12 and IL 13 mRNA in children with asthma. Methods Use of semi quantitative RT PCR, IL 12 and IL 13 mRNA in peripheral blood mononuclear cell (PBMC), as well as total IgE in serum from children with asthma, which is in the period of acute phase, were detected. Results Compared with control group, The expression level of IL 12 mRNA were decreased and that of IL 13 mRNA were increased in asthmatic children; The sicker the patient was, the lower expression of IL 12 mRNA, the higher expression of IL 13 mRNA; No matter how the IgE level was, there was significantly different between the expression of IL 12 and IL 13 mRNA. Conclusion IL 12 and IL 13 may be one of the factors causing bronchial chronic inflammation.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2002年第5期284-286,共3页
Chinese Journal of Laboratory Medicine
基金
浙江省医药卫生科学研究基金 (9812 )
关键词
儿童
哮喘
白细胞介素类
逆转录聚合酶链反应
免疫学
Interleukins
Reverse transcriptase polymerase chain reaction
Immunologic techniques
