摘要
大豆乳清是制备大豆分离蛋白时产生的副产物,带来的环境污染问题十分严重。为了利用这一废弃物资源并促进传统产业升级,本研究进一步探索了大豆乳清的营养功能。利用喷雾干燥方法得到大豆乳清粉,检测其蛋白质、膳食纤维、低聚糖、灰分及氨基酸组成等主要成分指标。将大豆乳清分别按0. 5%、1. 0%和2. 0%的添加量加入小鼠饮食,饲喂28 d后分析其对动物肠道菌群及其产生短链脂肪酸(SCFAs)的影响。结果表明:大豆乳清粉主要成分如下:灰分含量20. 9%,蛋白质含量20. 1%,低聚糖含量9. 68%,总膳食纤维含量5. 01%,脂肪含量3. 8%。与对照组相比,大豆乳清粉饲喂小鼠的肠道中厚壁菌门与拟杆菌门菌群比值显著降低,比值为0. 71～1. 20;另枝菌相对丰度增加,毛螺菌、拟普雷沃菌、拟杆菌、普雷沃菌相对丰度降低。不同剂量大豆乳清粉饲喂小鼠的肠道中总SCFAs含量显著提高,且SCFAs结构有明显不同。本研究结果可以为大豆乳清的高附加值利用提供理论依据和基础数据。
Soy whey(SW) is generated as a process waste while preparing soy protein isolate(SPI),causing environmentalpollution. In order to utilize this waste resource and promote the upgrading of traditional industry,this study further exploresthe nutritional function of SW. In this paper,Soy whey power(SWP) was obtained by fluidized drying,whose main compo-nents(including protein,dietary fiber,oligosaccharides and ash and amino acid composition and so on) were detected. SWPwas added to the diet of mice at the dosage of 0. 5%,1. 0% and 2. 0%,respectively. The effects of SWP on the intestinalmicroflora and the yields of short chain fatty acid(SCFAs) were analyzed after feeding for 4 weeks. The results showed that:In the SWP,the contents of ash,protein,oligosaccharide,total dietary fiber and fat were 20. 9%,20. 1%,9. 68%,5. 01%and 3. 8%,respectively. Compared with the control group,the ratio of Firmicutes and Bacteroides was significantly decreasedin the feces of mice fed SWP,as 0. 71-1. 20. The relative abundance of Alistipes increased,instead that of Lachnospira,Al-loprevotella,Bacteroides and Prevotella decreased. The yields of the total SCFAs were significantly promoted and the structureof SCFAs were significantly different in the feces of mice fed various doses of SWP. This study provides the theory evidenceand basic data for the high value-added utilization of SW.
作者
韩伟
庄绪会
张云鹏
陈园
王大为
张晓琳
HAN Wei;ZHUANG Xu-hui;ZHANG Yun-peng;CHEN Yuan;WANG Da-wei;ZHANG Xiao-lin(Academy of State Administration of Grain,Beijing 100037,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2019年第1期104-110,共7页
Soybean Science
基金
中央级公益性科研院所基本科研业务费专项资金(ZX1712)
关键词
大豆乳清
小鼠
肠道菌群
厚壁菌门
拟杆菌门
短链脂肪酸
Soy whey
Mice
Intestinal microflora
Firmicutes
Bacteroides
Short chain fatty acids(SCFAs)
