摘要
目的研究具有雌激素活性的环境污染物辛基酚对雌激素受体阳性的乳腺癌细胞MCF_7凋亡调控基因bcl_2mRNA表达的影响及与雌二醇作用的异同。方法分别以不同浓度的17β_雌二醇和辛基酚刺激体外培养的MCF_7细胞12～120h ,用RT_PCR方法测定细胞bcl_2mRNA的表达量。结果17β_雌二醇和辛基酚作用MCF_7细胞24h后bcl_2mRNA表达量显著升高,持续至120h ,较宽浓度范围的辛基酚均可引起bcl_2表达增高 ,以10-6 mol/L作用最强。结论辛基酚具有类似雌二醇的刺激MCF_7细胞bcl_2表达的作用 。
Objective To explore the effects of octylphenol, an environmental contaminant with estrogenic activity, on the expression of apoptosis regulator gene bcl-2 mRNA in human breast cancer cell line MCF-7 and to compare its effects with 17β-estradiol (E 2). Methods MCF-7 cells were cultured in vitro and exposed to various concentrations of octylphenol and E 2 from 12 h to 120 h respectively. Total RNA was abstracted and the expression of bcl-2 mRNA was measured using reverse transcription polymerase chain reaction (RT-PCR) approach. Results The expression of bcl-2 mRNA in MCF-7 cells increased significantly after exposure to octylphenol and E 2 24 h and the effects continued to 120 h. The stimulation effect on the expression of bcl-2 mRNA was induced by octylphenol with a wide range of concentrations, and the strongest effect was found at the concentration of 10-6 mol/L of octylphenol. Conclusion The results suggested that octylphenol had similar estrogenic activities as E 2 on regulating the expression of bcl-2 mRNA in MCF-7 cells, but the effect was weaker than that of E 2.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2002年第5期358-360,共3页
Journal of Environment and Health
基金
国家自然科学基金资助项目 (39800127)
