摘要
研究了鲫鱼幼体 (Carassiusauratus)在低浓度铅的长期 ( 4 0d)暴露实验中 ,鲫鱼抗氧化系统所产生的生理生化反应 .结果表明 ,在本实验的剂量范围内 ,铅对鲫鱼肝脏内过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD)的活性 (在暴露浓度达到 0 2mg·l- 1时 )表现为诱导作用 ;而铅对谷胱甘肽硫转移酶 (GST)、谷胱甘肽过氧化物酶 (GSH Px)的活性在低浓度时 ( 0 0 1mg·l- 1)被抑制 ,其中暴露浓度在 0 2mg·l- 1时GSH Px被进一步抑制 .在实验范围内 ,GSH含量无明显变化 .另外 ,对于低浓度铅的长期暴露 ,鲫鱼肝脏中的GST和GSH Px较CAT和SOD敏感 ,可考虑作为水生生态系统中铅低浓度长期暴露的一种生物检测指标 .
The aim of this work was to study the effects of trace lead under long term exposure on fish antioxidant system,such as the activities of SOD,CAT,GSH-Px,GST and the content of GSH.During 40 days exposure,we found that the activities of SOD and CAT increased significantly when the concentration of Pb 2+ reach 0.2 mg·l -1 ,whereas the activities of GST and GSH-Px decreased significantly at 0.01mg·l -1 Pb 2+ and GSH-Px activity decreased again at 0.2 mg·l -1 Pb 2+ ,no obvious change of GSH content in the fish liver was found.These results suggest that GST and GSH-Px are more sensitive than CAT and SOD to low dose lead exposure,and they could be served as bio-monitoring index of low concentration lead exposure in aquatic ecosystem.
出处
《环境化学》
CAS
CSCD
北大核心
2002年第5期485-489,共5页
Environmental Chemistry
基金
教育部博士点基金资助项目 (编号 2 0 0 0 0 2 82 4 )
