摘要
目的 观察血管内皮生长因子 (VEGF)在子宫腺肌病患者子宫组织的表达 ,探讨子宫腺肌病患者血管生成活性的变化。方法 手术切除子宫患者 62例 ,其中子宫腺肌病 3 2例 (腺肌病组 ) ,无症状子宫肌瘤 3 0例 (肌瘤组 )。用免疫组织化学方法检测VEGF在正常子宫内膜、子宫肌层、腺肌病病灶 (包括异位内膜及其周围子宫肌层 )或肌瘤组织中的表达。采用MPIAS 5 0 0彩色图像处理系统进行图像分析。结果 腺肌病组子宫内膜腺上皮VEGF组织学评分 ,增殖期 ( 9 6± 1 4)分 ,分泌期( 11 7± 1 6)分 ;肌瘤组增殖期 ( 8 3± 1 7)分 ,分泌期 ( 10 2± 1 5 )分。两组分别比较 ,差异有显著性 (P<0 0 5 )。两组子宫内膜腺上皮VEGF组织学评分均于分泌期显著增加 (P <0 0 5 )。腺肌病组异位内膜腺上皮VEGF组织学评分 ,增殖期 ( 11 9± 1 8)分 ,分泌期 ( 13 0± 1 7)分 ,较在位内膜明显增加 (P<0 0 5 ) ,但无周期性变化。腺肌病组异位内膜周围子宫肌层VEGF组织学评分 ,增殖期 ( 9 5± 1 3 )分 ,分泌期 ( 8 7± 1 3 )分 ,较正常子宫肌层的增殖期 [( 4 8± 1 9)分 ]、分泌期 [( 4 5± 1 4)分 ]明显增加(P <0 0 1)。结论 腺肌病患者子宫内膜和异位内膜周围肌层VEGF表达显著增强 。
Objective To investigate the role of angiogenesis and expression of vascular endothelial growth factor (VEGF) in the pathophysiology of adenomyosis of the uterus. Methods The study included 32 patients with histologically proven adenomyosis and 30 patients with asymptomatic leiomyoma of the uterus. Immunohistochemical staining was used to detect VEGF expression in different parts of the uterus. A computerized morphometric study on the VEGF expression was performed. Results The VEGF H-Score of glandular cells in the endometrium of adenomyosis uterus [proliferative phase (9.6±1.4), secretory phase (11.7±1.6)] was much higher than that of leiomyoma uterus [proliferative phase (8.3±1.7),secretory phase (10.2±1.5)] (P<0.05), and increased in the secretory phase (P<0.05). Compared with eutopic endometrium, the VEGF H-Score in ectopic endometrium [proliferative phase (11.9±1.8), secretory phase (13.0±1.7)] increased significantly (P<0.05), but showed no cyclic change (P>0.05). The VEGF H-Score of myometrium around the ectopic lesions [proliferative phase (9.5±1.3), secretory phase (8.7±1.3)] was higher than that of normal myometrium [proliferative phase (4.8±1.9), secretory phase (4.5±1.4)] (P<0.01). Conclusion The angiogenesis activity of the endometrium and myometrium of adenomyosis uterus was markedly increased.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2002年第9期539-541,共3页
Chinese Journal of Obstetrics and Gynecology
