摘要
本试验旨在研究饲粮中添加沙葱粉或复合益生菌制剂对杜寒杂交羊瘤胃发酵参数和瘤胃液菌群多样性的影响。选取体重相近的杜寒杂交羊30只,随机分为3组,每组10只,各组饲喂饲粮如下:对照组,基础饲粮;沙葱组,基础饲粮+沙葱粉(沙葱粉添加量为饲粮干物质的0.46%);复合益生菌组,基础饲粮+复合益生菌制剂(复合益生菌制剂添加量为饲粮干物质的4.35%)。饲喂90 d后,随机从每组羊群中选取体重相近的试验羊4只,屠宰后采集瘤胃液,检测瘤胃发酵参数,并运用高通量测序方法检测瘤胃菌群多样性。结果表明:1)与对照组相比,复合益生菌组瘤胃液乙酸浓度显著提高(P<0.05),沙葱组瘤胃液乙丙比下降(P>0.05),复合益生菌组瘤胃液乙丙比则升高(P>0.05)。2)复合益生菌组瘤胃液香农指数显著高于对照组(P<0.05),而沙葱组瘤胃液香农指数虽高于对照组,但差异不显著(P> 0.05)。3)在前10种菌门中,沙葱组各菌门相对丰度与对照组存在差异,但差异均不显著(P>0.05);复合益生菌组拟杆菌门、疣微菌门和互养菌门的相对丰度显著高于对照组(P<0.05),而变形菌门的相对丰度显著低于对照组(P<0.05);在科水平上,沙葱组和复合益生菌组氨基酸球菌科的相对丰度均显著低于对照组(P<0.05)。由此得出,饲粮中添加沙葱粉或复合益生菌制剂均可以改变瘤胃发酵类型,并可不同程度地丰富瘤胃液菌群多样性。
The aim of this study was to evaluate the effects of dietary Allium mongolicum Regel ( AMR) powder or probiotic complex ( PC) preparation on ruminal fermentation parameters and rumen fluid bacterial diversity of Dorper × thin-tailed Han crossbred sheep. Thirty Dorper × thin-tailed Han crossbred sheep with similar body weight were randomly divided into 3 groups and each group had 10 sheep. The diets of the three groups were as follows: control group,basal diet only;AMR group,basal diet+ 0.46% AMR powder;PC group, basal diet+4.35% PC preparation. The adding amounts of AMR powder and PC preparation were according to the dry matter of the basal diet. After feeding 90 days,four sheep with similar body weight were randomly selected, the rumen fluid of every sheep was sampled after slaughter to detect ruminal fermentation parameters, and the rumen fluid bacterial diversity was determined high-throughput sequencing method. The results showed as follows: 1) compared with the control group,the concentration of acetic acid of rumen fluid in PC group was significantly increased ( P< 0.05) ,the acetate /propionate of rumen fluid in AMR group was lowed and that in PC group was increased,but the differences were not significant ( P>0.05) . 2) The Shannon index of rumen fluid in PC group was significantly lower than that in control group ( P<0.05) ,and that in AMR group was higher than that in control group,but the difference was not significant ( P>0.05) . 3) Within top 10 bacteria at phylum level,there were differences between control and AMR group in the relative abundance of each bacterial phylum,but the differences were not significant ( P>0.05);The relative abundance of Bacteroidetes, Verrucomicrobia and Synergistetes in PC group were significantly higher than those in control group ( P < 0.05) ,however,the relative abundance of Proteobucteria was significantly lower than that in control group ( P<0.05) . At family level,the relative abundance of Acidaminococcaceae in AMR group and PC group was significantly higher than that in control group ( P<0.05) . The results indicate that AMR powder and PC preparation supplementations can change the type of rumen fermentation,and make the rumen fluid bacterial diversity more abundant at different extents,respectively.
作者
丁赫
刘旺景
敖长金
哈斯额尔敦
李书仪
李英
张佐忠
DING He;LIU Wangjing;AO Changjin;Khas-Erdene;LI Shuyi;LI Ying;ZHANG Zuozhong(College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2019年第1期324-333,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
内蒙古自治区重大专项"中国-加拿大肉羊养殖及饲草料品种开发与利用"
关键词
沙葱粉
复合益生菌制剂
杜寒杂交羊
瘤胃发酵
菌群多样性
Allium mongolicum Regel powder
probiotic complex preparation
Dorper×thin-tailed Han crossbred sheep
rumen fermentation
bacterial diversity
