摘要
目的:检测HOXB4、PRDM16及HOXA9在急性髓系白血病(AML)患者骨髓中的表达水平,探讨其临床意义。方法:采用实时荧光定量PCR方法,检测40例初治或复发AML患者、9例完全缓解患者和19例对照者(非恶性血液病患者及健康供者)骨髓细胞HOXB4、PRDM16及HOXA9 mRNA表达水平,分析其与疾病特征、临床疗效之间关系。结果:初治或复发AML患者骨髓细胞中HOXB4、PRDM16和HOXA9表达水平均明显高于非恶性血液病患者(P<0.05);初治或复发AML患者HOXB4基因表达水平与骨髓白血病细胞比例成正相关(r=0.39),HOXB4、PRDM16和HOXA9基因的表达水平两两之间具有相关性;AML不同病程阶段(初诊、复发、缓解)HOXB4、PRDM16基因表达水平差异具有统计学意义;按预后危险度分层将初治及复发AML患者分为高、中、低危3组,3组之间HOXB4、PRDM16、HOXA9表达水平的差异无统计学意义;经2个疗程正规化疗,缓解组(CR/PR)患者治疗前的3个基因表达水平均低于未缓解组治疗前水平(P<0.05);上述3个基因低表达组缓解率均高于高表达组(P<0.05)。结论:HOXB4、PRDM16、HOXA9基因表达水平与AML患者肿瘤负荷、疗效和预后存在一定的关系。在初治和复发的AML患者骨髓细胞高表达,缓解后其表达水平下降,表达高者化疗效果差,缓解率低。
Objective:To investigate HOXB4,PRDM16 and HOXA9 gene expression in patients with acute myeloid leukemia(AML) and its clinical significance.Methods:Real-time quantitative PCR(RT-qPCR) with SYBR Green assay was used to detect the expression of HOXB4 PRDM16 and HOXA9 gene in AML patients(40 cases),the patients with complete remission(9 cases) and patients with non-malignant hematologic diseases as control(10 cases).The relationship between the expression levels of gene HOXB4,PRDM16,HOXA9 and clinical features was investigated by statistical analysis.Results:The gene expression levels of HOXB4,PRDM16,HOXA9 in newly diagnosed or relapsed AML patients were significantly higher than those in patients with non-malignant hematologic disease(P <0.05).It was observed that the expression of HOXB4 gene in newly diagnosed or relapsed patients positively correlates with leukemic blasts in bone marrow(r =0.39).The expression levels of HOXB4 PRDM16 and HOXA9 positively correlate with each other.There was statistical significance among gene expressions in different phases(newly diagnosed,relapse,remission).No correlation was observed between expression levels of HOXB4 PRDM16,HOXA9 and chromosome risk status.It was noticed that expression levels of HOXB4,PRDM16,HOXA9 genes were lower in the patients achieved remission after two courses of chemotherapy than those in the other.And high expression group of each gene had a lower remission rate than that in the low expression group.Conclusion:The expression level of HOXB4,PRDM16,HOXA9 genes and leukemic blasts somewhat correlate with curative effect and prognosis.The expression of HOXB4 PRDM16,HOXA9 genes is higher in newly diagnosed and relapsed leukemia patients,and lower in the patients acquired CR/PR.High expression of HOXB4 PRDM16,HOXA9 genes predicts an adverse prognosis.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2016年第2期326-331,共6页
Journal of Experimental Hematology
基金
山东省自然基金资助(ZR2009CM056)
