摘要
目的建立同时测定金果饮咽喉片中没食子酸、橙皮苷含量的方法。方法采用超高效液相色谱法。色谱柱为ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7 μm),流动相为乙腈(A)-0.1%磷酸水(B)(梯度洗脱),流速为0.40 m L/min,检测波长为280 nm,柱温为30℃,进样量为1 μL。结果没食子酸、橙皮苷检测进样量线性范围分别为0.048~0.480 μg、0.016~0.160 μg;检测限分别1.6、1.8 ng,定量限分别为7.5、5.6 ng;精密度、重复性试验的RSD≤3.0%。结论该方法快速、准确,可用于金果饮咽喉片中没食子酸、橙皮苷的含量测定。
Objective To establish a method for simultaneous determination of Gallic acid and hesperidin in Jinguoyin Throat Tablets. Methods Ultrahigh performance liquid chromatography was(HPLC)used. The chromatographic column was ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm), and the mobile phase was acetonitrile-0.1% phosphoric acid(gradient elution), the flow rate was 0.40 m L/min, the detection wavelength was 280 nm, the column temperature was 30℃, and the sample quantity was 1 μL. Results The linear range for detection of Gallic acid and hesperidin was 0.048-0.480,0.016-0.160μg respectively. The detection limit was 1.6 and 1.8 ng/g and the quantitative limit was 7.5 and 5.6 ng/g, respectively. The RSD of precision and repeatability test was less than 3.0%. Conclusion The method is rapid and accurate, and can be used for the determination of Gallic acid and hesperidin in Jinguoyin Yanghui tablets.
出处
《大医生》
2018年第4期88-89,共2页
Doctor
关键词
超高速液相色谱法
金果饮咽喉片
没食子酸
橙皮苷
ultra high speed liquid chromatography
jinguoyin pharynx tablet
gallic acid
hesperidin
