摘要
目的 :观察乳腺癌MCF 7细胞上白细胞介素 2受体 (IL 2R)α、β和γ链的表达、IL 2对MCF 7细胞增殖的作用及雌激素对三条链表达的影响。方法 :使用特异性IL 2R多克隆抗体以免疫细胞化学方法和流式免疫荧光法检测MCF 7细胞上IL 2R的表达 ,以MTT法及3 H TdR掺入法检测细胞增殖情况。结果 :MCF 7细胞上存在IL 2Rα、β、γ的免疫阳性物质 ,其中IL 2Rγ的表达要强于IL 2Rα、β的表达 ;10 -6mol/L浓度的雌二醇可促进IL 2Rα、β的阳性细胞数及IL 2Rγ的免疫阳性物质的含量 ;IL 2在 10 0U/ml至 10 0 0U/ml的浓度范围内可显著促进MCF 7细胞的增殖。结论 :MCF 7细胞上存在IL 2R且其表达受雌二醇的调节 ,IL 2可能通过IL 2R影响MCF
Aim: To investigate the expression of interleukin 2 receptors (IL 2Rs) on MCF 7 cells, estradiol's regulation of IL 2Rs expression and the influence of IL 2 on the proliferation of MCF 7 cells. Methods: Immunocytochemistry and flow cytometric analysis were used to investigate the expression of interleukin 2 receptors (IL 2Rs) by using of specific IL 2R polyclonal antibodys; MTT method and 3 H TdR incorporation method were used to examine the changes of proliferation of MCF 7 cells. Results: IL 2Rα,β,γ like immunoreactive substances can be found on MCF 7 cells and the IL 2Rγ immunostaining was more strong than the other two. Estradiol of 10 -6 mol/L can increase the percentage of immunoreactive cells of IL 2Rα?β and the expression of IL 2Rγ. Exogenous addition of recombinant IL 2 of 100 U/ml to 1 000 U/ml can significantly increase the proliferation of MCF 7 cells. Conclusion:MCF 7 cell can express IL 2R and estradiol can regulate their expression, IL 2 can influence the proliferation of MCF 7 cells.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2002年第3期287-290,共4页
Chinese Journal of Applied Physiology
