摘要
目的:研究石斛多糖对D-半乳糖诱导衰老小鼠学习记忆损伤的保护作用。方法:通过每天皮下注射(sc)D-半乳糖120 mg·kg-160 d诱导小鼠学习记忆损伤模型。观察石斛多糖(80,160,320 mg·kg-1)灌胃(ig)60 d后对小鼠学习记忆功能的影响。结果:Morris水迷宫实验、跳台实验(step-down test)和避暗实验(step-through test)显示石斛多糖能够明显改善D-半乳糖诱导的小鼠学习记忆损伤。此外,石斛多糖能够降低脑组织和血浆中丙二醛(malondialdehyde,MDA)的含量以及升高超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)和Na+-K+-ATP酶的活力。结论:石斛多糖能够保护D-半乳糖诱导的衰老损伤,其作用机制可能与石斛多糖的抗氧化损伤有关。
Objective:To investigate the effects of Dendrobium polysaccharides on D-galactose-induced learning and memory deficits in aging mice. Methods:Adult mice were given subcutaneous injection of D-galactose(120 mg·kg-1)daily for 60 d to produce agingrelated syndrome,such as learning and memory deficits and alterations in tissue redox status. Dendrobium polysaccharides(80,160 and 320 mg·kg-1)were administrated by gavage daily for 60 d. Results:Dendrobium polysaccharides significantly ameliorated D-galactose-induced cognitive deficits,as evidenced by the significant performance improvement in the Morris water maze,step-down and step-through tests. Dendrobium polysaccharides also prevented the elevation of malondialdehyde(MDA)in the brain and plasma and restored the antioxidant enzyme activities to normal levels,including superoxide dismutase(SOD),glutathione peroxidase(GSH-PX)and Na+-K+-ATP ase. Conclusions:Dendrobium polysaccharides is effective against D-galactose-induced aging and this effect appears to be due to,at least in part, its antioxidant activities.
出处
《神经药理学报》
2012年第2期1-6,共6页
Acta Neuropharmacologica
基金
国家自然科学基金项目(No.30660206,No.30960447)
贵州省科技厅重点项目(No.20051001)
贵州省中医药管理局(No.C262)
关键词
石斛多糖
D-半乳糖
学习记忆损伤
抗氧化损伤
Dendrobium polysaccharides
D-galactose
learning and memory deficits
antioxidant activity
