摘要
从杭州市郊菜园表现严重花叶、矮化症状的绿花椰菜病株上获得一病毒分离物Ｃ－９３１。汁液摩擦接种６科２１种植物，Ｃ－９３１可侵染其中的６科１９种；体外抗性测定表明Ｃ－９３１的稀释限点（ＤＥＰ）为１０￣（－２），致死温度（ＴＩＰ）为５５－６０℃，２５℃体外存活期（ＬＩＶ）为４ｄ；提纯病毒粒体球形，平均直径２８ｎｍ；在琼脂双扩散反应中Ｃ－９３１能与ＣＭＶ抗血清呈阳性反应；ＳＤＳ－ＰＡＧＥ测得其衣壳蛋白亚基分子量为２９ｋＤ；用ＣＦ－１１纤维素柱提取受侵植物组织中ｄｓＲＮＡ，电泳鉴定表明共有５个核酸组分，长度（ｋｂ）分别为３．３，３．０，２．２，０．９和０．３５．根据上述性状，Ｃ９３１被鉴定为黄瓜花叶病毒，且该分离物含卫星ＲＮＡ。
A virus isolate named C-931 was obtained from broccoli(Brassica oleiacea L. var.italica Planch) in the suburb of Hangzhou in 1993.21 species in 6 families were tested by mechani-cal inoculation,among them 19 species in 6 families were infected. The thermal inactivation point(TIP)was 55-60℃),dilution end point (DEP)10￣(-2) and longevity in vitro(LIV)4 days at 25C. The virus particles were isometric with an average diameter of about 28 nm. Agarose doublediffussion test showed that it could react strongly with the antiserum of CMV. The subunit of thecoat protein was measured by SDS-PAGE and the molecular weight was about 29 kD; Double-stranded RNA analysis indicated that the isolate had five RNA components with the length about3. 3 kb,3. 0 kb,2. 0 kb,0.9kb and 0.35 kb, respectively. On the basis of these characteristics,the isolate C-931 was identified as a broccoli isolate of CMV with a satellite RNA. The fieid inves-tigation indicated that CMV commonly infected broccoli.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
1995年第6期610-614,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省科委资助
