摘要
DNA芯片技术是近年来迅速发展起来的分子生物学技术 ,它将成千上万个寡核苷酸固定在厘米大小的硅片上 ,将待测的材料用荧光素或同位素标记 ,在DNA芯片上与探针杂交 ,通过激光共聚焦显微镜对芯片进行扫描获取杂交探针的荧光信号。该技术在人类基因组计划、DNA测序、转录情况分析、基因诊断等方面得到广泛应用。毒理学是生命科学的重要分支之一 ,DNA芯片技术在毒理学中也有潜在的广泛应用前景。本文就DNA芯片技术的原理 ,制作方法 ,信号检测 。
DNA chip is a new molecular biology technology rapidly developed in recent years. It fixes thousands of oligonucleotide on a silicon chip of 1 cm 2, hybridizes the measuring materials marked with fluorescein or isotope with probe in DNA chip,and gets the fluorescent signal of hybridized probe through the scan of confocalmicroscope. This technology has been extensively used in HGP, DNA sequencing, transcript analysis, gene diagnosis etc. Toxiology is an important branch of life science. DNA chip can be extensively used in the toxiological field. This paper reviewed the DNA chip technology in mechanism, manufacturing method, signal check, advantages and application prospect in toxiology.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2002年第5期387-390,共4页
Space Medicine & Medical Engineering
