摘要
运用细胞生物学、生物化学等手段 ,观察鉴定分离提取的牡蛎天然活性肽BPO 1对人胃腺癌细胞的生物学效应 .实验结果表明 ,BPO 1能有效抑制人胃腺癌BGC 82 3细胞增殖活动 ,细胞生长受到抑制 ,分裂指数下降 .细胞周期检测出现凋亡峰 .光镜观察显示经BPO 1处理后的BGC 82 3细胞失去原有的恶性表型 ,表现出明显不同于对照组细胞 .
BPO-1, eluted from oyster cells (Saccostrea Cucullata), treat the human Gastric Adenocarcinoma BGC-823 cells in vitro for investigating the biological effects by the methods of cell biology, biochemistry and molecular biology. The results revealed that the proliferation of BGC-823 cells were inhibited apparently after being treated with BPO-1, the growth of cells were inhibited, the cell mitotic index was declined obviously. Flow cytometry analysis showed BPO-1 could induce the emergence of the peak of apoptosis. Light microscopy showed that BGC-823 cells, which induced with BPO-1, had lost their original malignant phenotype which were different from those of untreated cells but were similar to those of apoptotic cells, and the abilities of AKPase were declined. The results showed that BPO-1 could inhibit the proliferation of the human gastric adenocarcinoma BGC-823 cells and act as a apoptosis inducer in its antitumor effects.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第5期618-622,共5页
Journal of Xiamen University：Natural Science
基金
福建省自然科学基金 (F0 10 0 0 10 )资助项目
