摘要
设计、合成了 1对特异性引物 ,以PRV MinA和PRV YueA株的病毒基因组为模板扩增gE的抗原表位基因 ,均获得了约 5 6 0bp的特异性产物 .将PCR产物克隆到pUCm T载体上 ,构建重组质粒pUCMAgE和pUCYAgE .经菌落PCR和质粒酶切鉴定后 ,将目的基因进行序列测定 .结果表明 :目的基因均由 5 5 8bp组成 ,编码 186个氨基酸 ;核酸序列和推导的氨基酸序列的同源性分析表明 ,PRV YueA和PRV MinA株具有相对较低的同一性 ,分别为 96 9%和92 5 % .
A pair of primers were designed and synthesized. Using the whole genomes of strain PVR MinA and PRV YueA as templates,specific products were obtained as expected by PCR amplification and cloned into pUCm T vector.Recombinants were confirmed by colony PCR and restriction enzyme digestion.The inserts were sequenced,and the results revealed that each of both inserts composed of 558 neucleotides,coding for 186 amino acids.Comparison of the target genes showed 96 9% nuleotide sequence homology and 92.5% amino acid sequence homology between strain PRV YueA and PRV MinA,which is relatively low.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2002年第4期71-74,共4页
Journal of South China Agricultural University
基金
广东省科技攻关资助项目 (ZKM0 35 0 7N)
国家自然科学基金资助项目 (39770 0 33)
