摘要
目的 探讨复合型完全脱蛋白骨的细胞相容性。方法 将猪肋骨用理化方法制得复合型完全脱蛋白骨（CFDB）,对CFDB理化性能进行检测,又将其与人胎骨膜成骨细胞体外复合培养,以了解其细胞相容性及细胞毒性。结果CFDB的主要成份为羟基磷灰石,蛋白质含量几乎为零,仍具有原骨组织骨盐支架的三维多孔网状孔隙结构系统,对复合培养的人胎骨膜成骨细胞的形态特征、ALP活性、细胞周期、倍体水平无有害影响。结论CFDB具有原骨组织的网状孔隙结构系统,具有良好的细胞相容性,可望用于成骨细胞的支架材料。
Objective To study the cellular compatibility of composite fully deproteinised bone(CFDB) Methods CFDB was processed by physical and chemical methods with pig ribs and its properties were examined, Then its cellular compatibility and cytotoxicity were examined by osteoblasts culture in vitro. Results The main ingredients of CFDB was hydroxyapatite;it hardly had any protein,and it had natural pore system which possessed pore network system of original bone. It has no bad affect on cell feature,alkaline phosphatase activity and cell cycle of human embryonic periosteal-derived osteoblasts composited with CFDB. Conclusion CFDB possessed original bone natural pore system and well cellular compatibility,and it could be used as scalfolds of osteoblasts.
出处
《中国骨伤》
CAS
2002年第10期597-599,共3页
China Journal of Orthopaedics and Traumatology
基金
云南省自然科学基金资助项目(2000C0010R)
