期刊文献+

桑叶总黄酮对糖尿病大鼠小肠双糖酶的抑制作用 被引量:112

Inhibitory effect of total flavonoids from mulberry tree leaf on small intestine disaccharidases in diabetic rats
原文传递
导出
摘要 目的 观察桑叶总黄酮对糖尿病大鼠的降糖作用及其对大鼠双糖酶活性的影响。方法 糖尿病大鼠灌胃给桑叶总黄酮 ,观察给药前后血糖变化 ;剥离小肠刷状缘膜 ,匀浆后加桑叶总黄酮 ,测定对双糖酶活性的抑制率 ;大鼠小肠注入麦芽糖 ,30、6 0、12 0分钟后取门静脉、外周静脉血 ,测门 外周静脉血糖差。结果 桑叶总黄酮使糖尿病大鼠血糖降低 ;对蔗糖酶、麦芽糖酶、乳糖酶活性抑制率依次达 6 8.0 %、4 7.1%、2 7.8% ;使注入麦芽糖后门 外周静脉血糖浓度差降低。结论 桑叶总黄酮通过抑制大鼠小肠双糖酶活性有显著的降血糖作用。 Objective To observe the hypoglycemic effect of total flavonoids from mulberry tree leaf (MTF) on diabetic rats and the effect of MTF on disaccharidases from rats. Methods Diabetic rats were treated with MTF, and then the change of blood glucose of these rats was observed; the brush border membrane from the small intestine of rats was stripped and homogenized, which was incubated with MTF and disaccharides, and the inhibitory rate of MTF on disaccharidases was determined. Blood samples from portal and peripheral veins were separately collected at 30, 60, 120 min after maltose solution was infused into small intestine in vivo, then the difference of blood glucose concentration between portal and peripheral veins was determined. Results MTF exerted a hypoglycemic effect on diabetic rats by inhibition of disaccharidases, the inhibitory rate of sucrase, maltase and lactase was 68.0%, 47.1%, 27.8% respectively, the difference of blood glucose concentration between portal and peripheral veins was also reduced. Conclusion The hypoglycemic effect of MTF on diabetic rats is probably via the inhibitory effect on small intestine disaccharidases in rats.
出处 《中华内分泌代谢杂志》 CAS CSCD 北大核心 2002年第4期313-315,共3页 Chinese Journal of Endocrinology and Metabolism
关键词 降血糖机制 桑叶总黄酮 糖尿病 大鼠 小肠 双糖酶 Flavones Folium Mori Diabetes mellitus, experimental Disacharidases
  • 相关文献

参考文献4

二级参考文献3

  • 1李良.论糖尿病与肝[J]新中医,1985(08).
  • 2冯仁丰.用葡萄糖氧化酶-过氧化物酶试剂制品测定血清葡萄糖[J]上海医学,1980(12).
  • 3钱荣立.糖尿病的饮食治疗[J].中国实用内科杂志,1991,12(12):621-623. 被引量:4

共引文献150

同被引文献1417

引证文献112

二级引证文献1225

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部