摘要
肿瘤的基因 放射治疗是近年来发展起来的新技术 .分别于体外和体内检测含辐射敏感启动子和mIL 12基因的真核表达载体 (pNEgr mIL 12 )的生物学活性 .体外经酶联免疫吸附试验 (enzymelinkedimmunosorbentassay ,ELISA)法检测转染 pNEgr mIL 12重组质粒的COS 7和B16细胞可经辐射诱导mIL 12p70表达 ,于 1 5～ 2 0Gy照射后表达增高最明显 ,COS 7细胞于照后4h达峰值 ,B16细胞的表达水平随照射后时间延长而逐渐增高 ;pNEgr mIL 12重组质粒联合电离辐射治疗小鼠移植肿瘤 ,单次或多次注射pNEgr mIL 12重组质粒 ,联合局部照射能够抑制小鼠移植肿瘤生长 ,与单纯照射组比较肿瘤生长速度减慢 ,瘤重降低 ,尤以多次给予质粒治疗组效果明显 .
The expression of mIL 12 p70 in the supernatant of cultured COS 7 and B16 melanoma cells transfected with pNEgr mIL 12 and the tumor growth rate in mice after gene radiotherapy with pNEgr mIL 12 plasmid and different doses X irradiation were observed. The expression of mIL 12 p70 in the supernatant of COS 7 and B16 melanoma cells were most prominent after X irradiation with 1 5～2 0 Gy, and doses as low as 0 05 Gy also showed a stimulatory effect. Time course studies showed that the expression of mIL 12 p70 in the supernatant of COS 7 cells reached its peak at 4 h after irradiation and a progressive increase in expression of mIL 12 p70 in the supernatant of B16 melanoma cells was observed over the study period of 72 h. In vivo , the injection of pNE mIL12 recombinat plasmid into tumor followed by local X irradiation one or three times could inhibit the growth of B16 melanoma implanted in C57BL/6J mice and the surviving days of tumor bearing mice was delayed. It will provide an experimental basis for planning effective clinical gene radiotherapy of cancer.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2002年第5期790-795,共6页
Progress In Biochemistry and Biophysics
