摘要
探讨新生儿基因细胞色素P4 5 0 2E1(CYP2E1) 5′端RsaⅠ多态性和对氧磷酶 2 (二乙基对硝基苯磷酸酯酶 2 )基因 311位点 (PON2 311)多态性对早产的影响。采用横断面调查方法 ,使用统一的调查表 ,由安庆市各县医院对入院分娩孕妇及其单胎、活产、早产和对照新生儿进行调查 ,共得到有效样本 194个母亲 -新生儿对。单因素分析结果显示 :CYP2E1野生纯合子基因型 (cut/cut)与突变纯合子基因型 (uncut/uncut) /杂合子基因型 (cut/uncut)比较 ,对早产的影响不具有统计学意义。而PON2Ser311Ser纯合子基因型与Cys311Cys纯合子基因型 /Cys311Ser杂合子基因型比较 ,对早产的影响具有显著的统计学意义。进一步分析CYP2E15′端RsaⅠ位点多态性和PON2 311位点多态性是否存在交互作用 ,结果显示 :CYP2E1野生纯合子基因型和PON2Ser311Ser纯合子基因型这一组合与参照组比较 ,对早产的影响具有显著的统计学意义。基因CYP2E15′端RsaⅠ位点多态性与新生儿早产不相关 ,但基因PON2 311位点多态性与新生儿早产相关 ,且CYP2E15′端RsaⅠ位点多态性和PON2 311位点多态性之间对早产的影响存在交互作用。
The objective is to investigate whether Rsa Ⅰ polymorphism in the 5′ flanking region of CYP2E1 and PON2311 polymorphism in neonates are associated with preterm. Using standard questionnaires, 194 singleton live born mother neonate pairs (including preterm cases and term controls) were investigated by the trained field workers with cross sectional survey at the hospitals in Anqing, Anhui Province, China. Epidemiological and clinical data and blood samples were obtained from 194 mother neonate pairs. CYP2E1 homozygous wild type ( cut/cut) is not associated with a shortened gestation among neonates, compared with CYP2E1 homozygous mutant type ( uncut/uncut) / CYP2E1 heterozygote ( cut/uncut) before and after adjustment confounders. However, PON2 Ser311Ser homozygote is significantly associated with a shortened gestation among neonates. When Rsa Ⅰ polymorphism in the 5′ flanking region of CYP2E1 and PON2311 polymorphism were considered jointly, a significant shortened gestation was observed among neonates with the combined genotype of CYP2E1 homozygous wild type and PON2 Ser311Ser homozygote. In conclusion, Rsa Ⅰ polymorphism inthe 5′ flanking region of CYP2E1 in neonates is not associated with preterm, however, PON2311 polymorphism in neonates is significantly associated with preterm. Furthermore, the gene interaction between Rsa Ⅰ polymorphism in the 5′ flanking region of CYP2E1 and PON2311 polymorphism in neonates is significantly associated with preterm.
基金
国家计生委科技进步重点项目 (990 1) ~~
