摘要
12 %的蔗糖浓度、 0 5 %甘氨酸、溶菌酶酶解 1h,是金色链霉菌原生质体制备的较优条件。采用麸皮再生培养基替代R2YE再生培养基 ,原生质体再生率、生长及筛选效果得到明显改善。P buffer介导的质粒转化效率高于T buffer,33%的PEG1 0 0 0是质粒转化金色链霉菌原生质的最适浓度。
12% sucrose, 0.5% glycin and 1 hour enzymolysis of lysozyme were the optimal conditions for preparation of Streptomyces aureofaciens protoplast. The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium. P-buffer was better than T-buffer in plasmid transformation process. 33% PEG1000 was the fit concentrations for plasmid transformation.
出处
《微生物学通报》
CAS
CSCD
北大核心
2002年第5期14-17,共4页
Microbiology China
基金
福建省科委资助项目 (No. 99 H 46)
福州大学科技发展基金项目 (No XKJ (QD) -0 1 3 0 )
