摘要
以鸟苷产生菌BacillussubtilisAJ2 0 66为生产菌株 ,采用 5 0L自控发酵罐与摇瓶培养相结合的联动优化方法对鸟苷发酵进行了研究。谷氨酸钠对鸟苷发酵比较重要 ,培养基中加入 1 %的谷氨酸钠可使 5 0L罐最终产苷达 31 49g/L。次黄嘌呤 (Hx)作为前体可以直接用于鸟苷合成 ,发酵后期加入 0 2 %的Hx,可使 5 0L罐最终产苷达 33 2 4g/L。
Bacillus subtilis AJ2066 was used to yield guanosine. The optimization of guanosine fermentation was studied by means of flask coupled with 50L auto-controlled fermentor. Monosodium glutamate is important to guanosine fermentation. The yield reached 31.49g/L in 50L fermentor with the medium supplemented with 1% monosodium glutamate. Hypoxanthine (Hx) can be used to synthesize guanosine directly as precursor. The yield reached 33.24g/L in 50L fermentor by supplementing 0.2% hypoxanthine during final stage of fermentation.
出处
《微生物学通报》
CAS
CSCD
北大核心
2002年第5期65-69,共5页
Microbiology China
基金
上海市曙光计划资助项目 (No 0 1SG2 8)
关键词
鸟苷
发酵
联动优化
菌株
培养基
Guanosine,Fermentation,Coupled-optimization
