摘要
目的 评价异丙酚对肿瘤坏死因子α(TNF-α)诱导的人脐静脉内皮细胞(HUVECs)凋亡的影响.方法3~4代人脐静脉内皮细胞于融合状态,随机分为5组:①TNF-α组(P0+TNF-α);②12.5μmol/L异丙酚和TNF-α组(P12.5+TNF-α);③25μmol/L异丙酚和TNF-α组(P25+TNF-α);④50μmol/L异内酚和TNF-α组(P50+TNF-α);⑤100μmol/L异丙酚和TNF-α组(P100+TNF-α);TNF-α终末浓度为2000U/ml,各组先加入不同浓度的异丙酚孵育30min后再加入TNF-α共同培养24h。用DNA原位缺口末端标记(TUNEL)技术检测细胞凋亡指数(AI),并用透射电镜观察细胞形态学改变。结果 肿瘤坏死因子组(P0+TNF-α)可见较多凋亡细胞,不同浓度的异丙酚预处理后再加入肿瘤坏死因子的各组(P12。5+TNF-α、P25+TNF-α、P50+TNF-α、P100+TNF-α细胞凋亡指数与肿瘤坏死因子组(P0+TNF-α)比较,均有明显下降(P<0.05或P<0.01),且随异丙酚浓度的升高,AI逐渐减小,内皮细胞损伤明显减轻。结论 临床相关浓度的异丙酚可抑制TNF-α诱导的内皮细胞凋亡。
Objective To investigate the protective effects of propofol in clinical relevant concentration on TNF-induced apoptosis in endothelial cells derived from human umbilical vein (HUVECs) .Methods The third and fourth passages of the cultured endothelial cells were divided into 5 groups. 0, 12.5, 25, 50 or 100 μmol/L propofol was added to the cultured cells respectively and the cells were incubated for 30 min. Then TNF-α was added to the cultured cells (the final concentration of TNF-α was 2000 U.ml-1 ) which was incubated for 24h. Apoptosis was detected by using 'terminal deoxynucleotidyl transferase (tdt )-mediated deoxyuridine triphosphate (dUTP )-biotin nick end-labelling' (TUNED . Morphologic changes were examined by means of electron microscope.Results Apoptosis expression was very high in control group (no propofol was added) . The process can be inhibited by pre-treatment with propofol (P< 0.05 or < 0.01 ) . With increasing concentrations of propofol, apoptosis index and morphologic changes were significantly decreased in TNF-injured cells. Conclusion Propofol in clinical relevant concentration has significant protective effects against TNF-induced apoptosis in HUVECs.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2002年第10期619-621,共3页
Chinese Journal of Anesthesiology