摘要
目的 :探讨麦粒灸对类风湿关节炎大鼠TNF α、IFN γ的影响。方法 :以佐剂性关节炎(AA)作为类风湿关节炎大鼠模型 ,随机分为正常对照组、模型组、麦粒灸治疗组、麦粒灸加去肾上腺组、麦粒灸加假手术组 ,麦粒灸“肾俞”、“足三里” ,检测TNF α、IFN γ含量变化。结果 :正常对照组、模型组、麦粒灸治疗组、麦粒灸加去肾上腺组、麦粒灸加假手术组大鼠血清TNF α水平分别是1 2 .1 3± 9.0 1 pg/mL、46.75± 1 6.77pg/mL、1 3 .2 5± 8.64 pg/mL、3 1 .88± 2 4.3 4pg/mL、6.88±4.1 7pg/mL ;正常对照组、模型组、麦粒灸治疗组、麦粒灸加去肾上腺组、麦粒灸加假手术组大鼠血清IFN γ含量分别为 470 .2 3± 1 0 2 .0 1ng/L、1 45.58± 46.65ng/L、451 .1 4± 98.64ng/L、2 53 .88±75.44ng/L、42 5.97± 1 1 4.1 7ng/L。结论 :麦粒灸下调AA大鼠血清TNF α水平和上调AA大鼠血清IFN γ水平 ,二者均有赖于肾上腺的完整。
Objective: To explore the effect of moxa-grain moxibustion on serum tumor necrosis factor (TNF-α) and interferon-γ(IFN-γ) levels in rheumatoid arthritis (RA) rats. Methods: 49 SD rats were randomly divided into control group, model group, moxibustion group, moxibustion+adrenalectomy group, moxibustion+ pseudo-operation group with 10 cases in each group except adrenalectomy group (n=9). RA model was established by injecting complete Freund's adjuvant (CFA, 0.1 mL) into the rats' right paw. Moxibustion (with moxa grain) was applied to 'Zusanli'(ST 36) and 'Shenshu'(BL 23) once daily, with 6 days being a therapeutic course, 2 courses altogether. Serum TNF-α and IFN-γ were assayed using Elisa double antibody sandwich method. Results: Serum TNF-α contents of control, model, moxibustion, adrenalectomy and pseudo-operation groups were 12.13±9.01 pg/mL, 46.75±16.77 pg/mL, 13.25±8.64 pg/mL, 31.88±24.34 pg/mL and 6.88± 4.17 pg/mL separately; while serum IFN-γ contents of the above-mentioned groups were 470.23±102.01 ng/L, 145.58±46.65 ng/L, 451.14±98.64 ng/L, 253.88±75.44 ng/L and 425.97± 114.17 ng/L respectively. That is, in RA model group, serum TNF-α content increased significantly whereas IFN-γ decreased remarkably in comparison with control group (P<0.01, 0.01), after moxibustion serum TNF-α and IFN-γ levels returned to normal nearly. In adrenalectomy group, both TNF-α and IFN-γ levels were between those of model and moxibustion group, meaning attenuation of the effect of moxibustion after adrenalectomy. No significant difference was found between moxibustion group and pseudo-operation group in these two indexes. Conclusion: Moxibustion may significantly lower serum TNF-α level and raise IFN-γ level in RA rats. The effect of moxibustion in regulating TNF-α and IFN-γ levels has a close relation with the intact of adrenal gland.
出处
《针刺研究》
CAS
CSCD
2002年第3期201-204,共4页
Acupuncture Research
基金
国家自然科学基金资助课题 (NO .39870 95 6 )
关键词
类风湿关节炎
细胞免疫
麦粒灸
Rheumatoid arthritis Cellular immunity Moxibustion therapy
