摘要
目的 分析正常肺、支气管组织和肺癌组织的激光诱发自体荧光光谱特征 ,探讨利用激光诱发自体荧光区分正常肺、支气管组织与肺癌组织的可行性。方法 收集肺癌手术标本 42例 ,每一标本的不同部位即肿瘤组织和正常肺组织及正常支气管组织分别平均测定 4～ 5条光谱曲线 ,共测得 196个部位的激光诱发自体荧光光谱图 ,其中癌组织 74个 ,肺组织 6 7个 ,正常支气管组织 5 5个。使用三倍频YAG激光 (波长 35 5nm)和光学多道分析仪测定各部位的激光诱发自体荧光光谱 ,读出每条光谱曲线的主峰波长 ,峰值强度 ,5 80nm和 6 0 0nm处的荧光强度。结果 ①正常肺、支气管组织和肺癌组织的主峰位置不同 ,正常肺组织主峰位置(4 71 6± 5 2 )nm较肺癌 (4 6 1 9± 4 3)nm及支气管 (4 6 3 6± 3 6 )nm的主峰位置向右偏移。②肺癌组织的荧光相对强度 (2 42 2 7± 434 9)大于正常肺组织 (1485 4± 46 2 1) ;而支气管的荧光相对强度最高 (5 1376± 8332 )。③正常肺组织在 5 40～ 5 6 0nm及 5 80～ 6 0 0nm处有两个次峰 ,正常支气管组织在 5 80nm与 6 0 0nm表现为一平台 ,而肺癌组织平滑下降 ,正常肺组织在 5 80nm与 6 0 0nm的荧光相对强度比为 0 831± 0 177;正常支气管组织为 1 0 41± 0 117,肺癌为 1 2 6 9± 0 147(P
Objective To approach the regularity of Laser-induced fluorescence(LIF) of Normal Lung and Lung Cancer and study the feasibility of spectroscopy in the differentiation between normal lung tissue and lung cancer. Methods The LIF spectra of normal lung and lung cancer in 42 surgical speci-mens were examined with a detecting system which consists of an YAG laser(wave length 355 nm)and an optical multichannel analyzer(OMA),pathological examinations were done in every testing spots. Results① The location of the principal spectral peaks of the normal lung and lung cancer are different, there is a red shift in the peak emission wave length in the normal lung tissue(471.6±5.2)nm than that of lung cancer(461.9±4.3)nm and bronchus(463.6±3.6) nm; ②The relative fluorescence intensity of the lung cancer(24 227±4 349)is higher than that of the normal lung(14 854±4 621), but the bronchus is the highest(51 376±8 332); ③In the normal lung tissue spectrum, there are two subsidiary maxima located clearly at (540～560) nm and (580～600) nm. The cancer tissue spectrum shows an almost structureless and smoother profile with no subsidiary maxima. The mean relative fluorescence intensity ratio values at 580 nm and 600 nm were 0.831±0.177 for normal lung tissue, 1.041±0.117 for normal bronchus and 1.269±0.147 for lung cancer(P<0.001). Conclusion There is a significant different fluorescence spectrum between normal lung?normal bronchus and lung cancer. And it is feasible to differentiate lung cancer from normal lung tissue and normal bronchus with the use of LIF spectroscopy.
出处
《海军总医院学报》
2002年第3期145-147,共3页
Journal of Naval General Hospital of PLA
基金
全军科研基金资助 (0 1Q0 2 8)
