摘要
用非洲菊试管苗叶片作外植体获得了有再分化能力的愈伤组织和正常的再生植株。在适宜培养基上叶片切块的出愈率和出芽率最高可达 96 %和 90 %;小苗 4周增殖倍数为 10 .85 ,生根率 99%,最快 4周就能从叶片切块成苗。试管苗叶片快繁最适培养基为 :起始MS +6 BA 3 .0mg·L-1+NAA 0 .1mg·L-1;增殖MS +6 BA 3 .0mg·L-1+NAA 0 .2mg·L-1;生根 1/ 2MS +IAA 1.0mg·L-1。用作外植体的试管苗以 3～ 4叶期为佳 ,叶片切块以 4mm× 4mm为宜。
Regenerable calli and plantlets were obtained from leaf and petiole segments of Gerbera in vitro plantlets and those regenerated plantlets were morphologically normal.The rate of callus formation and rate of shoot formation on optimum medium were as high as 96% and 90% separately.Four weeks'in vitro multiple times for those plantlets was 10.85 while root formation rate was 99% during root inducing.The earliest plantlets could be obtained in 4 weeks.Optimal media for micropropagation after optimization experiments were MS+6-BA 3.0mg·L -1+NAA 0.1mg·L -1 for induction;MS+6-BA 3.0mg·L+NAA 0.2mg·L -1 for multiplication of plantlets;1/2 MS+IAA 1.0mg·L -1 for root formation.In vitro plantlets used for explants was at the stage with 3-4 leaves and suitable size of cut leaf segments was about 4mm×4mm.
出处
《园艺学报》
CAS
CSCD
北大核心
2002年第5期493-494,共2页
Acta Horticulturae Sinica
