摘要
目的建立RP-HPLC同时测定射干药材中芒果苷、射干苷、野鸢尾苷、鸢尾黄素、鸢尾甲黄素B、鸢尾甲黄素A、野鸢尾黄素、白射干素及次野鸢尾黄素共9种成分含量的方法。方法采用LeapsilTM C18色谱柱(100 mm×2.1 mm,3μm),流动相为乙腈-0.1%甲酸水溶液梯度洗脱,检测波长265 nm,流速0.5 mL/min,柱温40℃。结果芒果苷、射干苷、野鸢尾苷、鸢尾黄素、鸢尾甲黄素B、鸢尾甲黄素A、野鸢尾黄素、白射干素、次野鸢尾黄素的线性范围分别为0.214 0~2.568μg(r=0.999 5)、0.437 0~5.244μg(r=0.999 3)、0.460 0~5.520μg(r=0.999 9)、0.078 40~0.940 8μg(r=0.999 6)、0.138 0~1.656μg(r=0.999 3)、0.051 00~0.612 0μg(r=0.997 5)、0.113 0~1.356μg(r=0.999 9)、0.051 63~0.619 6μg(r=0.999 8)、0.151 0~1.812μg(r=0.999 9),平均加样回收率分别为97.73%、96.81%、97.78%、97.55%、96.86%、98.60%、97.77%、98.04%、97.89%,RSD分别为0.7%、1.1%、2.3%、2.1%、1.3%、1.4%、2.3%、1.6%、1.9%。应用该方法测定了5批射干药材中9种成分的含量。结论该方法准确、可靠,可为射干药材的质量控制提供参考依据。
Objective To develop an RP-HPLC method for simultaneous determination of mangiferin, tectoridin,iridin, tectorigenin, iristectorigenin B, iristectorigenin A, irigenin, dichotomin and irisflorentin in Belamcandae Rhizoma. Methods Separation was carried out on an LeapsilTM C18 column (100 mm×2.1 mm, 3 μm) with an isocratic mobile phase consisting of acetonotrile and formic acid at a flow rate of 0.5 mL/min; The detection wavelength was set at 265 nm; the column temperature was 40 ℃. Results The linear ranges of mangiferin,tectoridin, iridin, tectorigenin, iristectorigenin B, iristectorigenin A, irigenin, dichotomin and irisflorentin were 0.214 0–2.568 μg (r=0.999 5), 0.437 0–5.244 μg (r=0.999 3), 0.460 0–5.520 μg (r=0.999 9), 0.078 40–0.940 8 μg (r=0.999 6),0.138 0–1.656 μg (r=0.999 3), 0.051 00–0.612 0 μg (r=0.997 5), 0.113 0–1.356 μg (r=0.999 9), 0.051 63–0.619 6 μg(r=0.999 8) and 0.151 0–1.812 μg (r=0.999 9), respectively. The average recoveries were 97.73%, 96.81%, 97.78%,97.55%, 96.86%, 98.60%, 97.77%, 98.04% and 97.89%, respectively; the relative standard deviations were 0.70%,1.1%, 2.3%, 2.1%, 1.3%, 1.4%, 2.3%, 1.6% and 1.9%, respectively. This method was used to determine the contents of nine active ingrients in 5 batches of Belamcandae Rhizoma. Conclusion The method is accurate and reliable, which can be used for the quality control of Belamcandae Rhizoma.
作者
尤献民
邹桂欣
邸子真
李国信
YOU Xian-min;ZOU Gui-xin;DI Zi-zhen;LI Guo-xin(Liaoning Academy of Chinese Medicine, Shenyang 110034, China)
出处
《中国中医药信息杂志》
CAS
CSCD
2017年第1期82-86,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81273927)
国家临床重点专科建设项目(2013年)
国家中医药管理局临床中药学重点学科(2009年)
辽宁中医药大学杏林学者青蓝工程(2013年)
关键词
射干
活性成分
反相高效液相色谱法
含量测定
Belamcandae Rhizoma
active ingredients
RP-HPLC
content determination
