摘要
目的评价荧光定量聚合酶链式反应(PCR)、金标法、酶联免疫法检测不孕妇女生殖道沙眼衣原体感染的应用效果。方法 57例确诊为生殖道沙眼衣原体感染的不孕妇女作为研究对象,使用一次性无菌采样拭子采集患者宫颈口分泌物,分别采用荧光定量PCR、金标法、酶联免疫法检测沙眼衣原体,对比三种检测方法的阳性率。结果荧光定量PCR(96.49%)与酶联免疫法(92.98%)的沙眼衣原体阳性检出率比较,差异无统计学意义(χ~2=0.704,P>0.05);荧光定量PCR及酶联免疫法的沙眼衣原体阳性检出率均高于金标法(82.46%),差异均具有统计学意义(χ~2=5.961、2.931,P<0.05)。结论与金标法比较,荧光定量PCR和酶联免疫法检测不孕妇女生殖道沙眼衣原体感染的准确性更高,两种方法均可为该疾病患者临床治疗方案的制定提供具有高度客观性的参考依据。
Objective To evaluate the application effect of fluorescent quantitation polymerase chain reaction(PCR),golden-standard method and euzymelinked immunosorbent assay in detection of genital chlamydia trachomatis infection in infertility women.Methods A total of57infertility women with confirmed genitalchl amydia trachomatis infection as study subjects,and their cervical secretions were collected with disposable sterile swab sampling.Detection were made on chlamydia trachomatis by fluorescent quantitation PCR,goldenstandardmethod and euzymelinked immunosorbent assay,and the positive rate of three detection methods was compared.Results There was no statistically significant difference in positive detection rate in chlamydia trachomatis between fluorescent quantitation PCR(96.49%)and euzymelinked immunosorbent assay(92.98%)(χ2=0.704,P>0.05).Fluorescent quantitation PCR and euzymelinked immunosorbent assay had higher positive detection rate in chlamydia trachomatis than golden-standard method(82.46%),and the difference had statistical significance(χ2=5.961,2.931,P<0.05).Conclusion Compared with golden-standard method,fluorescent quantitation PCR and euzymelinked immunosorbent assay provides higher accuracy rate in detection of genital chlamydia trachomatis infection in infertility women,and both methods can provide highly objective reference for establishment of clinical treatment regimens for patients with this disease.
作者
吴乔羽
WU Qiao-yu(Department of Clinical Laboratory, Dongguan City Zhongtang Hospital, Dongguan 523220, China)
出处
《中国现代药物应用》
2017年第6期39-40,共2页
Chinese Journal of Modern Drug Application
关键词
不孕妇女
生殖道沙眼衣原体感染
荧光定量聚合酶链式反应
金标法
酶联免疫法
Infertility women
Genital chlamydia trachomatis infection
Fluorescent quantitation polymerase chain reaction
Golden-standard method
Euzymelinked immunosorbent assay
