摘要
采用超高效液相色谱-串联质谱法结合Qu ECh ERS(quick,easy,cheap,effective,rugged,safe)前处理方法,研究温州蜜柑人工接种链格孢菌(Alternaria alternate)后,病斑部位全果、非病斑部位果皮和果肉中腾毒素(tentoxin,Ten)、链格孢酚(alternariol,AOH)、交链格孢酚单甲醚(alternariol monomethyl ether,AME)、交链孢烯(altenuene,ALT)和细交链格孢菌酮酸(tenuazonic acid,Te A)5种链格孢霉毒素的产生和分布规律。结果表明,病斑部位全果、非病斑部位果皮和果肉中的链格孢霉毒素含量随着病斑直径的扩大,不同链格孢霉毒素的变化趋势不同。Te A、Ten、AME和AOH在所有部位中均有检出,其中Te A检出含量最高,在病斑部位的含量范围为3.05×10~3~55.88×10~3μg/kg,在非病斑部位的含量范围为65.35~40.68×10~3μg/kg;Ten、AME和AOH在病斑部位的含量范围分别为69.16~373.94、22.63~1 395.82μg/kg和8.18~689.19μg/kg;非病斑部位的含量范围分别为0~67.56、0~195.96μg/kg和0~301.91μg/kg;ALT在非病斑部位果肉中未检出,但在非病斑部位的果皮和病斑部位的全果中均有检出,含量最高达16.61μg/kg。研究表明,由于柑橘感染链格孢菌后产生的毒素会从发病部位扩散到健康部位累积,因此,在鲜食加工和风险评估中应引起关注和重视。
An utra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method was stablished using quick,easy,cheap,effective,rugged,and safe(QuEChERS)pre-treatment to investigate the production and distribution of five Alternaria mycotoxins including tentoxin(Ten),alternariol(AOH),alternariol monomethyl ether(AME),altenuene(ALT)and tenuazonic acid(TeA)in whole fruits,healthy pulp and healthy peel of citrus inoculated with Alternaria alternate.The results demonstrated that the contents of various Alternaria mycotoxins produced in whole fruits,healthy pulp and healthy peel changed differently with increasing lesion diameter.TeA,Ten,AME and AOH were detected in all tested parts of the fruit.Among these,the average concentration of TeA was the highest,ranging from3.05×103to55.88×103μg/kg and from65.35to40.68×103μg/kg in lesion and non-lesion areas,respectively.The concentrations of Ten,AME and AOH in the infected location were in the range of69.16?373.94,22.63?1395.82and8.18?689.19μg/kg,respectively,while in the health location,the values were in the range of0?67.56,0?195.96and0?301.91μg/kg,respectively.ALT was detected in both healthy peel and infected whole fruits(up to16.61μg/kg)rather than healthy pulp.These results indicate that Alternaria mycotoxins produced in infected citrus can rapidly spread from the infected to the healthy parts,resulting in the accumulation of a large amount of mycotoxins in citrus.Therefore,we should pay more attention to this problem in fresh food processing and risk assessment to ensure consumer safety.
作者
蒋黎艳
龚蕾
盖智星
刘雁雨
马良
赵其阳
王成秋
焦必宁
JIANG Liyan;GONG Lei;GE Zhixing;LIU Yanyu;MA Liang;ZHAO Qiyang;WANG Chengqiu;JIAO Bining(Laboratory of Quality and Safety Risk Assessment for Citrus Products, Ministry of Agriculture, Citrus Research Institute,Chinese Academy of Agricultural Sciences, Chongqing 400712, China;College of Food Science, Southwest University,Chongqing 400715, China;Quality Supervision and Testing Centre for Citrus and Seedling, Ministry of Agriculture,Chongqing 400712, China;National Citrus Engineering Research Center, Chongqing 400712, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2017年第8期251-257,共7页
Food Science
基金
国家重点基础研究发展计划(973计划)项目(2013CB127803)
国家自然科学基金青年科学基金项目(31301476)
国家现代农业(柑桔)产业技术体系建设专项(CARS-27)
国家农产品质量安全风险评估重大专项(GJFP2015004
GJFP2016004)
中央高校基本科研业务费专项(XDJK2013B035)
关键词
温州蜜柑
链格孢霉毒素
超高效液相色谱-串联质谱
分布规律
Satsuma mandarin
Alternaria mycotoxins
ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
distribution pattern
